Determination of advanced glycation endproducts in cooked meat products

Advanced glycation endproducts (AGES), a pathogenic factor implicated in diabetes and other chronic diseases, are produced in cooked meat products. The objective of this study was to determine the AGE content, as measured by N epsilon-carboxymethyllysine (CML) levels, in cooked chicken, pork, beef and fish (salmon and tilapia) prepared by three common cooking methods used by U.S. consumers: frying, baking, and broiling. The CML was detected in all the cooked samples, but the levels were dependent on types of meat, cooking conditions, and the final internal temperature. Broiling and frying at higher cooking temperature produced higher levels of CML, and broiled beef contained the highest CML content (21.8 mu g/g). Baked salmon (8.6 mu/g) and baked tilapia (9.7 mu g/g) contained less CML as compared to the other muscle food samples. (C) 2014 Elsevier Ltd. All rights reserved.