Detection of genetic variability in stygobitic isopods using RAPD markers

被引：4
|
作者：
Baratti, M
Bazzicalupo, M
De Filippo, C
Messana, G
机构：
[1] Univ Florence, Dipartimento Biol Anim & Genet Leo Pardi, I-50125 Florence, Italy
[2] CNR, Ctr Studio Faunist & Ecol Trop, I-50125 Florence, Italy
来源：
CRUSTACEANA | 1999年 / 72卷
关键词：
D O I：
10.1163/156854099503672
中图分类号：
Q17 [水生生物学];
学科分类号：
071004 ;
摘要：
Three species of the stygobitic isopod genus Stenasellus have been described from Italy: Stenasellus racovitzai, S. nuragicus, and S. assorgiai. Recent electrophoretic data suggest the presence of more than one species in the "racovitzai"-group. In order to clarify the degree of genetic variability and the evolutionary trends in the "racovitzai"-group, we have analysed three natural populations currently known from Italy and Corsica, by the Random Amplified Polymorphic DNA (RAPD) technique. RAPD has proven to be a rapid and valid method for the detection of intra- and interspecific genetic polymorphism in Crustacea and to be suitable for the analysis of DNA of preserved samples. This technique was carried out to generate RAPD fingerprints from the populations of the five localities. The molecular data obtained by RAPD markers confirm previously obtained morphological and electrophoretic results.
引用
收藏
页码：625 / 634
页数：10
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