Imaging photosensitizer distribution and pharmacology using multiphoton microscopy

被引：10

作者：

Wachter, E ^{[1
]}

Dees, C ^{[1
]}

Harkins, J ^{[1
]}

Fisher, W ^{[1
]}

Scott, T ^{[1
]}

机构：

[1] Photogen Inc, Knoxville, TN 37931 USA

来源：

OPTICAL DIAGNOSTICS OF LIVING CELLS V | 2002年 / 4622卷

关键词：

microscopy; imaging; multiphoton; MPE; femtosecond; photosensitizer;

D O I：

10.1117/12.468334

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Multiphoton microscopy is a powerful tool for imaging sub-cellular distribution of luminescent compounds present in living cells. We have used this tool to study the distribution and pharmacology of photosensitizers in tissue and tissue culture. Murine hepatoma tumor cells dosed with a photosensitizer were briefly photoactivated, then imaged for periods up to several hours. Using the photosensitizer Rose Bengal with green light activation, nearly immediate photolytic release of lysosomal enzymes resulted in catastrophic cell destruction within 5-30 minutes. The magnitude and rapidity of this response is markedly different than that observed with other photosensitizer agents, and is consistent with in vivo studies illustrating that Rose Bengal is capable of causing extremely rapid destruction of treated tumors.

引用

页码：112 / 118

页数：7

共 8 条

[1] 2-PHOTON LASER SCANNING FLUORESCENCE MICROSCOPY [J].