Effects of Candida albicans infection on defense effector secretion by human oral mucosal epithelial cells

被引：10
作者：
Huang, Fan [1 ]
Song, Yuefeng [1 ]
Chen, Wei [1 ]
Liu, Qin [1 ]
Wang, Qiong [2 ,3 ]
Liu, Weida [2 ,3 ]
Wang, Xiang [1 ]
Wang, Wenmei [1 ]
机构：
[1] Nanjing Univ, Med Sch, Nanjing Stomatol Hosp, Dept Oral Med, 30 Zhongyang Rd, Nanjing 210008, Jiangsu, Peoples R China
[2] CAMS, Inst Dermatol, Dept Mycol, Nanjing, Peoples R China
[3] PUMC, Jiangsu Key Lab Mol Biol Skin Dis & STIs, Nanjing, Jiangsu, Peoples R China
来源：
ARCHIVES OF ORAL BIOLOGY | 2019年 / 103卷
关键词：
Candida albicans; Defense; Effector molecule; Secretion; Oral mucosal epithelial cells; IMMUNOGLOBULINS; MECHANISMS; BETA; INFLAMMATION; EXPRESSION; PROTECTION; VIRULENCE; ENCODES; SALIVA;
D O I：
10.1016/j.archoralbio.2019.05.013
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Objective: The aim of this study was to investigate the effects of Candida albicans on the production of defense effector molecules by human oral mucosal epithelial cells in vitro. Design: Immortalized human oral mucosal epithelial (Leuk-1) cells and C. albicans strain 5314 were cocultured at different cell-to-C. albicans ratios. The viability of Leuk-1 cells was determined by MTT and RTCA measurements. The secretory levels of multiple defense effector molecules were determined by Enzyme-linked immunosorbent assay (ELISA). Results: Our results indicated that C. albicans significantly decreased the secretion of IgG, cystatin C, lactoferrin, and TGF-beta 1 in a dose-dependent manner and remarkably reduced the production of IgA independent of the cell to-C. albicans ratio. However, C. albicans clearly enhanced the secretion of IgM, galectin-3, P-selectin, granzyme B and perforin. Conclusion: These results suggest that C. albicans may exert a regulatory role in the defense response of oral mucosal epithelial cells by altering secretory levels of defense effector molecules.
引用
收藏
页码：55 / 61
页数：7
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