The protective effect of grape seed procyanidin extract against cadmium-induced renal oxidative damage in mice

被引：70

作者：

Chen, Qing ^{[1
,2
,3
]}

Zhang, Rong ^{[3
]}

Li, Wei-min ^{[1
,2
]}

Niu, Yu-jie ^{[3
]}

Guo, Hui-cai ^{[3
]}

Liu, Xue-hui ^{[3
]}

Hou, Yu-chun ^{[3
]}

Zhao, Li-juan ^{[3
]}

机构：

[1] Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Reprod Med, Xian 710049, Shanxi, Peoples R China

[2] Xi An Jiao Tong Univ, Coll Med, Dept Biochem & Mol Biol, Xian 710061, Shanxi, Peoples R China

[3] Hebei Med Univ, Sch Publ Hlth, Dept Toxicol, Shijiazhuang 050017, Hebei, Peoples R China

来源：

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2013年 / 36卷 / 03期

关键词：

Cadmium; Renal damage; Grape seed procyanidin extract; Oxidative stress; LIPID-PEROXIDATION; MITOCHONDRIAL PATHWAY; INDUCED APOPTOSIS; RAT-LIVER; STRESS; BLOOD; NEPHROTOXICITY; MECHANISMS; TOXICITY; EXPRESSION;

D O I：

10.1016/j.etap.2013.07.006

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

As an important environmental pollutant, cadmium (Cd) can lead to serious renal damage. Grape seed procyanidins extract (GSPE), a biological active component of grape seed, has been shown to possess antioxidative effects. Here, we assessed the protective effect of GSPE on Cd-induced renal damage using animal experiment. After 30 days, the oxidative damage of kidney was evaluated through measurement of superoxide dismutase (SOD), glutathione peroxidation (GSH-Px) and malondialdehyde (MDA). Since, oxidative stress could lead to apoptosis, the renal apoptosis was measured using flow cytometer. Moreover, the expression of apoptosis-related protein Bax and Bcl-2 was analyzed by immunohistochemistry and Western blot. The results showed that Cd led to the decrease of SOD and GSH-Px activities, and the increase of MDA level, induced renal apoptosis. However, the coadministration of GSPE attenuated Cd-induced lipid peroxidation, and antagonized renal apoptosis, probably associated with the expression of Bax and Bcl-2. These data suggested that GSPE has protective effect against renal oxidative damage induced by Cd, which provide a potential natural chemopreventive agent against Cd-poisoning. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：759 / 768

页数：10

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