UHRF1, a modular multi-domain protein, regulates replication-coupled crosstalk between DNA methylation and histone modifications

被引：105

作者：

Hashimoto, Hideharu ^{[1
]}

Horton, John R. ^{[1
]}

Zhang, Xing ^{[1
]}

Cheng, Xiaodong ^{[1
]}

机构：

[1] Emory Univ, Sch Med, Dept Biochem, Atlanta, GA 30322 USA

来源：

EPIGENETICS | 2009年 / 4卷 / 01期

基金：

美国国家卫生研究院;

关键词：

DNA methylation; histone modifications; base flipping; SRA domain; inter-domain interactions; SRA DOMAIN; LINEAR DIFFUSION; STRUCTURAL BASIS; BINDING; RECOGNITION; BASE; NP95; METHYLTRANSFERASE; MECHANISM; COMPLEX;

D O I：

10.4161/epi.4.1.7370

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytosine methylation in DNA is a major epigenetic signal, and plays a central role in propagating chromatin status during cell division. However the mechanistic links between DNA methylation and histone methylation are poorly understood. A multi-domain protein UHRF1 (ubiquitin-like, containing PHD and RING finger domains 1) is required for DNA CpG maintenance methylation at replication forks, and mouse UHRF1-null cells show enhanced susceptibility to DNA replication arrest and DNA damaging agents. Recent data demonstrated that the SET and RING associated (SRA) domain of UHRF1 binds hemimethylated CpG and flips 5-methylcytosine out of the DNA helix, whereas its tandom tudor domain and PHD domain bind the tail of histone H3 in a highly methylation sensitive manner. We hypothesize that UHRF1 brings the two components (histones and DNA) carrying appropriate markers (on the tails of H3 and hemimethylated CpG sites) ready to be assembled into a nucleosome after replication.

引用

页码：8 / 14

页数：7

共 46 条

[1] The interaction of the SRA domain of ICBP90 with a novel domain of DNMT1 is involved in the regulation of VEGF gene expression [J].

Achour, M. ;

Jacq, X. ;

Ronde, P. ;

Alhosin, M. ;

Charlot, C. ;

Chataigneau, T. ;

Jeanblanc, M. ;

Macaluso, M. ;

Giordano, A. ;

Hughes, A. D. ;

Schini-Kerth, V. B. ;

Bronner, C. .

ONCOGENE, 2008, 27 (15) :2187-2197

[2] A piRNA pathway primed by individual transposons is linked to de novo DNA methylation in mice [J].

Aravin, Alexei A. ;

Sachidanandam, Ravi ;

Bourc'his, Deborah ;

Schaefer, Christopher ;

Pezic, Dubravka ;

Toth, Katalin Fejes ;

Bestor, Timothy ;

Hannon, Gregory J. .

MOLECULAR CELL, 2008, 31 (06) :785-799

[3] Recognition of hemi-methylated DNA by the SRA protein UHRF1 by a base-flipping mechanism [J].

Arita, Kyohei ;

Ariyoshi, Mariko ;

Tochio, Hidehito ;

Nakamura, Yusuke ;

Shirakawa, Masahiro .

NATURE, 2008, 455 (7214) :818-U12

[4] Structural basis for recognition of hemi-methylated DNA by the SRA domain of human UHRF1 [J].

Avvakumov, George V. ;

Walker, John R. ;

Xue, Sheng ;

Li, Yanjun ;

Duan, Shili ;

Bronner, Christian ;

Arrowsmith, Cheryl H. ;

Dhe-Paganon, Sirano .

NATURE, 2008, 455 (7214) :822-U13

[5] Flipping duplex DNA inside out -: A double base-flipping reaction mechanism by Escherichia coli MutY adenine glycosylase [J].

Bernards, AS ;

Miller, JK ;

Bao, KK ;

Wong, I .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (23) :20960-20964

[6] UHRF1 plays a role in maintaining DNA methylation in mammalian cells [J].

Bostick, Magnolia ;

Kim, Jong Kyong ;

Esteve, Pierre-Olivier ;

Clark, Amander ;

Pradhan, Sriharsa ;

Jacobsen, Steven E. .

SCIENCE, 2007, 317 (5845) :1760-1764

[7] Structural basis for the methylation state-specific recognition of histone H4-K20 by 53BP1 and Crb2 in DNA repair [J].

Botuyan, Maria Victoria ;

Lee, Joseph ;

Ward, Irene M. ;

Kim, Ja-Eun ;

Thompson, James R. ;

Chen, Junjie ;

Mer, Georges .

CELL, 2006, 127 (07) :1361-1373

[8] Ankyrin for methylated lysines [J].

Brent, Michael M. ;

Marmorstein, Ronen .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2008, 15 (03) :221-222

[9]

Chen T, 2006, CURR TOP MICROBIOL, V301, P179

[10] Finding a basis for flipping bases [J].

Cheng, XD ;

Blumenthal, RM .

STRUCTURE, 1996, 4 (06) :639-645

← 1 2 3 4 5 →