Gibberella zeae chitin synthase genes, GzCHS5 and GzCHS7, are required for hyphal growth, perithecia formation, and pathogenicity

被引:71
作者
Kim, Jung-Eun [1 ,2 ]
Lee, Hyo-Jin [1 ,2 ]
Lee, Jungkwan [1 ,2 ]
Kim, Ki Woo [3 ]
Yun, Sung-Hwan [4 ]
Shim, Won-Bo [5 ]
Lee, Yin-Won [1 ,2 ]
机构
[1] Seoul Natl Univ, Dept Agr Biotechnol, Ctr Fungal Pathogenesis, Seoul 151921, South Korea
[2] Seoul Natl Univ, Dept Agr Biotechnol, Ctr Agr Biomat, Seoul 151921, South Korea
[3] Seoul Natl Univ, Natl Instrumentat Ctr Environm Management, Seoul 151921, South Korea
[4] Soonchunhyang Univ, Dept Med Biotechnol, Asan 336745, South Korea
[5] Texas A&M Univ, Dept Plant Pathol & Microbiol, College Stn, TX 77843 USA
关键词
Balloon-shaped hyphae; Cell wall rigidity; Fusarium graminearum; Intrahyphal hyphae; Septa formation; MOTOR-LIKE DOMAIN; FUNGUS FUSARIUM-OXYSPORUM; CLASS-V; ASPERGILLUS-NIDULANS; CLASS-I; COCHLIOBOLUS-HETEROSTROPHUS; FUNCTIONAL-ANALYSIS; FEMALE FERTILITY; CSMA; PATHOGENESIS;
D O I
10.1007/s00294-009-0258-6
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Gibberella zeae causes Fusarium head blight of cereal crops, and sexual spores of the fungus play an important role as primary inocula. We isolated a restriction enzyme-mediated integration (REMI) transformant, ZH431, of G. zeae with defects in perithecia formation and virulence. Integration of the REMI vector resulted in disruption of GzCHS7 gene, which encodes a putative class VII chitin synthase with high similarity to Fusarium oxysporum ChsVb. A second chitin synthase, GzCHS5, is adjacently located in a head-to-head configuration with GzCHS7, and its deduced protein sequence showed similarity with a class V chitin synthase in F. oxysporum. Neither Delta GzChs5 nor Delta GzChs7 mutants produced perithecia or caused disease on barley heads. Microscopic observation revealed that both mutants formed balloon-shaped hyphae and intrahyphal hyphae and that cell wall rigidity of the mutants was weaker than that of the wild-type strain. Transcription profiles of GzCHS5 and GzCHS7 were not altered in Delta GzChs7 and Delta GzChs5, respectively, suggesting that transcription regulations of the genes are independent of each other. Our results demonstrate that GzCHS5 and GzCHS7 are indispensable for perithecia formation and pathogenicity as well as normal septa formation and hyphal growth in G. zeae.
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页码:449 / 459
页数:11
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