Alginate beads containing pH-sensitive liposomes and glucose oxidase: glucose-sensitive release

被引：16

作者：

Hong, Yeon Ji ^{[1
,2
]}

Lee, Hyeon Yong ^{[1
,2
]}

Kim, Jin-Chul ^{[1
,2
]}

机构：

[1] Kangwon Natl Univ, Sch Biotechnol & Bioengn, Chunchon 200701, Kangwon Do, South Korea

[2] Kangwon Natl Univ, Inst Biosci & Biotechnol, Chunchon 200701, Kangwon Do, South Korea

来源：

COLLOID AND POLYMER SCIENCE | 2009年 / 287卷 / 10期

关键词：

Alginate bead; Glucose oxidase; Liposomes; Glucose-sensitive release; DELIVERY; BEHAVIOR; LIPIDS;

D O I：

10.1007/s00396-009-2084-2

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Egg PC (EPC) liposomes bearing a copolymer of N-isopropylacrylamide, methacrylic acid, and octadecylacrylate (P(NIPAM-co-MAA-co-ODA)) were prepared as pH-sensitive liposomes. They were embedded in glucose oxidase (GOD)-immobilized alginate beads. The ratio of EPC/GOD/alginate in the beads was 7.8:1.0:140.4, and the beads were added to glucose solutions so that the concentration of GOD was 0.0068 mg/ml. The enzymatic activity of the immobilized GOD was one fifth to half of that of native enzyme. As the glucose concentration increased from 0 to 400 mg/dl, the degree of calcein release increased from 17% to 75%. The acidification induced by the enzymatic reaction would be responsible for the glucose-triggered release.

引用

页码：1207 / 1214

页数：8

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