Establishing a reproducible approach to study cellular functions of plant cells with 3D bioprinting

被引：11

作者：

Van den Broeck, Lisa ^{[1
]}

Schwartz, Michael F. ^{[1
]}

Krishnamoorthy, Srikumar ^{[1
]}

Tahir, Maimouna Abderamane ^{[1
,2
]}

Spurney, Ryan J. ^{[1
,3
]}

Madison, Imani ^{[1
]}

Melvin, Charles ^{[1
]}

Gobble, Mariah ^{[1
]}

Nguyen, Thomas ^{[1
]}

Peters, Rachel ^{[1
]}

Hunt, Aitch ^{[1
]}

Muhammad, Atiyya ^{[1
]}

Li, Baochun ^{[4
]}

Stuiver, Maarten ^{[5
]}

Horn, Timothy ^{[2
]}

Sozzani, Rosangela ^{[1
]}

机构：

[1] North Carolina State Univ, Plant & Microbial Biol Dept & NC Plant Sci Initia, Raleigh, NC 27695 USA

[2] North Carolina State Univ, Mech & Aerosp Engn Dept, Raleigh, NC 27695 USA

[3] North Carolina State Univ, Elect & Comp Engn Dept, Raleigh, NC 27695 USA

[4] Innovat Ctr BASF, Morrisville, NC 27560 USA

[5] BASF Innovat Ctr, Technol Pk 101, B-9052 Zwijnaarde, Belgium

来源：

SCIENCE ADVANCES | 2022年 / 8卷 / 41期

基金：

美国国家科学基金会;

关键词：

PHYTOSULFOKINE-ALPHA; FERTILE PLANTS; REGENERATION; CULTURE; PROTOPLASTS; EXPRESSION; HYDROGELS; ENHANCER; TOOL;

D O I：

10.1126/sciadv.abp9906

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Capturing cell-to-cell signals in a three-dimensional (3D) environment is key to studying cellular functions. A major challenge in the current culturing methods is the lack of accurately capturing multicellular 3D environments. In this study, we established a framework for 3D bioprinting plant cells to study cell viability, cell division, and cell identity. We established long-term cell viability for bioprinted Arabidopsis and soybean cells. To analyze the generated large image datasets, we developed a high-throughput image analysis pipeline. Furthermore, we showed the cell cycle reentry of bioprinted cells for which the timing coincides with the induction of core cell cycle genes and regeneration-related genes, ultimately leading to microcallus formation. Last, the identity of bioprinted Arabidopsis root cells expressing endodermal markers was maintained for longer periods. The framework established here paves the way for a general use of 3D bioprinting for studying cellular reprogramming and cell cycle reentry toward tissue regeneration.

引用

页数：11

共 57 条

[1] Laterals take it better - Emerging and young lateral roots survive lethal salinity longer than the primary root in Arabidopsis [J].