The use of 2-dimensional mapping in medical research

被引：0

作者：

Nakagawa, Hiroaki ^{[1
]}

机构：

[1] Hitachi High Technol Corp, Hitachinaka, Ibaraki 3120057, Japan

来源：

TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY | 2009年 / 21卷 / 118期

基金：

日本科学技术振兴机构;

关键词：

HPLC; N-glycan; isomer analysis; biosynthesis pathway; medical research; N-ACETYLGLUCOSAMINYLTRANSFERASE-V; ALPHA-MANNOSIDASE-II; LINKED OLIGOSACCHARIDES; SWAINSONINE; RESISTANCE; CANCER; CELLS;

D O I：

10.4052/tigg.21.87

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Herein the importance of the 2-dimensional mapping (2-DM) method in medical research area is introduced. This is the most powerful method for the analysis of glycan isomers. Glycans are synthesized by specific enzymes, and the analysis of isomers can lead to the identification of further directly related enzymes. Therefore, structural analysis using 2-DM connects readily to gene or protein expression analysis. Further, glycan purification during 2-DM analysis is also useful for sample preparation for mass spectroscopic analyses. This review describes the application of 2-DM to three research areas, namely, the alpha-mannosidase II alternative pathway, drug resistance in cancer cells, and the diagnosis of bladder cancer.

引用

页码：87 / 94

页数：8

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