A selective and sensitive optical sensor for dissolved ammonia detection via agglomeration of fluorescent Ag nanoclusters and temperature gradient headspace single drop microextraction

被引:47
作者
Dong, Jiang Xue [1 ]
Gao, Zhong Feng [1 ]
Zhang, Ying [1 ,2 ]
Li, Bang Lin [1 ]
Li, Nian Bing [1 ]
Luo, Hong Qun [1 ]
机构
[1] Southwest Univ, Sch Chem & Chem Engn, Key Lab Luminescent & Real Time Analyt Chem South, Minist Educ, Chongqing 400715, Peoples R China
[2] Sichuan Univ Sci & Engn, Coll Chem & Pharmaceut Engn, Zigong 643000, Peoples R China
基金
中国国家自然科学基金;
关键词
Headspace single drop microextraction; Dissolved ammonia; Ag nanoclusters; Fluorescence; UV-vis spectrophotometry; GOLD NANOPARTICLES; AQUEOUS-SOLUTION; WASTE-WATER; STABILITY; SAMPLES; OXIDE; PROBE; IONS; FISH; DNA;
D O I
10.1016/j.bios.2016.11.062
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
In this paper, a simple sensor platform is presented for highly selective and sensitive detection of dissolved ammonia in aqueous solutions without pretreatment based on temperature gradient headspace single drop microextraction (HS-SDME) technique, and fluorescence and UV-vis spectrophotometry are utilized with the Ag nanoclusters (Ag NCs) functioned by citrate and glutathione as the probe. The sensing mechanism is based on the volatility of ammonia gas and the active response of Ag NCs to pH change caused by the introduction of ammonia. High pH can make the Ag NCs agglomerate and lead to the obvious decrease of fluorescence intensity and absorbance of Ag NCs solution. Moreover, the presented method exhibits a remarkably high selectivity toward dissolved ammonia over most of inorganic ions and amino acid, and shows a good linear range of 10-350 mu M (0.14-4.9 mg N L-1.) with a low detection limit of 336 nM (4.70 mu g NL-1) at a signal-to-noise ratio of 3. In addition, the practical applications of the sensor have been successfully demonstrated by detecting dissolved ammonia in real samples.
引用
收藏
页码:155 / 161
页数:7
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