Implementation of the CRISPR-Cas9 system in fission yeast

被引：140

作者：

Jacobs, Jake Z. ^{[1
]}

Ciccaglione, Keith M. ^{[1
]}

Tournier, Vincent ^{[1
]}

Zaratiegui, Mikel ^{[1
]}

机构：

[1] Rutgers State Univ, Dept Mol Biol & Biochem, Piscataway, NJ 08854 USA

来源：

NATURE COMMUNICATIONS | 2014年 / 5卷

关键词：

IN-VIVO; RNA;

D O I：

10.1038/ncomms6344

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Application of the CRISPR-Cas9 genome editing system in the model organism Schizosaccharomyces pombe has been hampered by the lack of constructs to express RNA of arbitrary sequence. Here we present expression constructs that use the promoter/leader RNA of K RNA (rrk1) and a ribozyme to produce the targeting guide RNA. Together with constitutive expression of Cas9, this system achieves selection-free specific mutagenesis with efficiencies approaching 100%. The rrk1 CRISPR-Cas9 method enables rapid and efficient genome manipulation and unlocks the CRISPR toolset for use in fission yeast.

引用

页数：5

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