RD26 mediates crosstalk between drought and brassinosteroid signalling pathways

被引：208

作者：

Ye, Huaxun ^{[1
,10
]}

Liu, Sanzhen ^{[2
,3
,11
]}

Tang, Buyun ^{[1
,12
]}

Chen, Jiani ^{[1
]}

Xie, Zhouli ^{[1
]}

Nolan, Trevor M. ^{[1
]}

Jiang, Hao ^{[1
]}

Guo, Hongqing ^{[1
]}

Lin, Hung-Ying ^{[2
]}

Li, Lei ^{[4
,5
]}

Wang, Yanqun ^{[4
,5
]}

Tong, Hongning ^{[6
]}

Zhang, Mingcai ^{[7
]}

Chu, Chengcai ^{[6
]}

Li, Zhaohu ^{[7
]}

Aluru, Maneesha ^{[8
]}

Aluru, Srinivas ^{[9
]}

Schnable, Patrick S. ^{[2
,3
]}

Yin, Yanhai ^{[1
]}

机构：

[1] Iowa State Univ, Dept Genet Dev & Cell Biol, Ames, IA 50011 USA

[2] Iowa State Univ, Dept Agron, Ames, IA 50011 USA

[3] Data2Bio LLC, Ames, IA 50011 USA

[4] Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02115 USA

[5] Harvard Med Sch, Dept Genet, Boston, MA 02115 USA

[6] Chinese Acad Sci, Inst Genet, Beijing 100101, Peoples R China

[7] China Agr Univ, Dept Agron, Coll Agron & Biotechnol, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China

[8] Georgia Inst Technol, Sch Biol, Atlanta, GA 30332 USA

[9] Georgia Inst Technol, Sch Computat Sci & Engn, Atlanta, GA 30332 USA

[10] Dupont Pioneer Inc, Johnston, IA 50131 USA

[11] Kansas State Univ, Dept Plant Pathol, Throckmorton Hall, Manhattan, KS 66506 USA

[12] Indiana Univ Sch Med, Dept Biochem & Mol Biol, Indianapolis, IN 46202 USA

来源：

NATURE COMMUNICATIONS | 2017年 / 8卷

基金：

美国国家科学基金会;

关键词：

REGULATED GENE-EXPRESSION; NAC TRANSCRIPTION FACTORS; ABIOTIC STRESS RESPONSES; RECEPTOR KINASE BRI1; ARABIDOPSIS-THALIANA; PLANT-GROWTH; TYROSINE PHOSPHORYLATION; PROTEIN; TRANSDUCTION; BIOSYNTHESIS;

D O I：

10.1038/ncomms14573

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Brassinosteroids (BRs) regulate plant growth and stress responses via the BES1/BZR1 family of transcription factors, which regulate the expression of thousands of downstream genes. BRs are involved in the response to drought, however the mechanistic understanding of interactions between BR signalling and drought response remains to be established. Here we show that transcription factor RD26 mediates crosstalk between drought and BR signalling. When overexpressed, BES1 target gene RD26 can inhibit BR-regulated growth. Global gene expression studies suggest that RD26 can act antagonistically to BR to regulate the expression of a subset of BES1-regulated genes, thereby inhibiting BR function. We show that RD26 can interact with BES1 protein and antagonize BES1 transcriptional activity on BR-regulated genes and that BR signalling can also repress expression of RD26 and its homologues and inhibit drought responses. Our results thus reveal a mechanism coordinating plant growth and drought tolerance.

引用

页数：13

共 70 条

[1] Reverse engineering and analysis of large genome-scale gene networks [J].

Aluru, Maneesha ;

Zola, Jaroslaw ;

Nettleton, Dan ;

Aluru, Srinivas .

NUCLEIC ACIDS RESEARCH, 2013, 41 (01) :e24

[2] Rice xa13 Recessive Resistance to Bacterial Blight Is Defeated by Induction of the Disease Susceptibility Gene Os-11N3 [J].

Antony, Ginny ;

Zhou, Junhui ;

Huang, Sheng ;

Li, Ting ;

Liu, Bo ;

White, Frank ;

Yang, Bing .

PLANT CELL, 2010, 22 (11) :3864-3876

[3] Characterization of brassinazole, a triazole-type brassinosteroid biosynthesis inhibitor [J].

Asami, T ;

Min, YK ;

Nagata, N ;

Yamagishi, K ;

Takatsuto, S ;

Fujioka, S ;

Murofushi, N ;

Yamaguchi, I ;

Yoshida, S .

PLANT PHYSIOLOGY, 2000, 123 (01) :93-99

[4] CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].

BENJAMINI, Y ;

HOCHBERG, Y .

JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1995, 57 (01) :289-300

[5] Synthesis of Hydroxylated Sterols in Transgenic Arabidopsis Plants Alters Growth and Steroid Metabolism [J].

Beste, Lisa ;

Nahar, Nurun ;

Dalman, Kerstin ;

Fujioka, Shozo ;

Jonsson, Lisbeth ;

Dutta, Paresh C. ;

Sitbon, Folke .

PLANT PHYSIOLOGY, 2011, 157 (01) :426-440

[6] Role of the Arabidopsis thaliana NAC transcription factors ANAC019 and ANAC055 in regulating jasmonic acid-signaled defense responses [J].

Bu, Qingyun ;

Jiang, Hongling ;

Li, Chang-Bao ;

Zhai, Qingzhe ;

Zhang, Jie ;

Wu, Xiaoyan ;

Sun, Jiaqiang ;

Xie, Qi ;

Li, Chuanyou .

CELL RESEARCH, 2008, 18 (07) :756-767

[7] Evaluation of statistical methods for normalization and differential expression in mRNA-Seq experiments [J].

Bullard, James H. ;

Purdom, Elizabeth ;

Hansen, Kasper D. ;

Dudoit, Sandrine .

BMC BIOINFORMATICS, 2010, 11

[8] Firefly luciferase complementation imaging assay for protein-protein interactions in plants [J].

Chen, Huamin ;

Zou, Yan ;

Shang, Yulei ;

Lin, Huiqiong ;

Wang, Yujing ;

Cai, Run ;

Tang, Xiaoyan ;

Zhou, Jian-Min .

PLANT PHYSIOLOGY, 2008, 146 (02) :368-376

[9] Antagonistic Regulation of Arabidopsis Growth by Brassinosteroids and Abiotic Stresses [J].

Chung, Yuhee ;

Kwon, Soon Il ;

Choe, Sunghwa .

MOLECULES AND CELLS, 2014, 37 (11) :795-803

[10] Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana [J].

Clough, SJ ;

Bent, AF .

PLANT JOURNAL, 1998, 16 (06) :735-743

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