Stemness and differentiation potential-recovery effects of sinapic acid against ultraviolet-A-induced damage through the regulation of p38 MAPK and NF-κB

被引：12

作者：

Hwang, Young Sun ^{[2
]}

Park, See-Hyoung ^{[3
]}

Kang, Mingyeong ^{[1
]}

Oh, Sae Woong ^{[1
]}

Jung, Kwangseon ^{[5
]}

Park, Yong Seek ^{[4
]}

Lee, Jongsung ^{[1
]}

机构：

[1] Sungkyunkwan Univ, Dept Genet Engn, Coll Biotechnol & Bioengn, Suwon 16419, Gyunggi Do, South Korea

[2] Eulji Univ, Dept Dent Hyg, Coll Hlth Sci, Seongnam City 13135, Gyunggi Do, South Korea

[3] Hongik Univ, Dept Bio & Chem Engn, Sejong City 30016, South Korea

[4] Kyung Hee Univ, Sch Med, Dept Microbiol, Seoul 02453, South Korea

[5] Skincure Life Sci Inst, Seongnam City 13216, Gyunggi Do, South Korea

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

关键词：

MIGRATION INHIBITORY FACTOR; SCAVENGING ACTIVITY; IN-VITRO; CELLS; PROLIFERATION; MIF; ACTIVATION; RADIATION; CYTOKINE; EXTRACTS;

D O I：

10.1038/s41598-017-01089-5

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ultraviolet A (UVA) irradiation exerts negative effects on stemness and differentiation potential of stem cells. This study aimed to explore the effect of sinapic acid on UVA-irradiation-induced damages to stemness and differentiation potential of human-adipose-tissue-derived mesenchymal stem cells (hAMSCs) and its UVA-antagonist mechanisms. Sinapic acid attenuated UVA-induced reduction in the proliferative potential and stemness by upregulating OCT4, SOX2, and NANOG. In addition, sinapic acid significantly recovered UVA-induced reduction in expression level of hypoxia-inducible factor (HIF)-1 alpha. The antagonist effect of sinapic acid against stemness damage was mediated by reduceing PGE(2) production through inhibition of p38 MAPK and NF-kappa B. Moreover, sinapic acid attenuated UVA-induced reduction in differentiation potential by downregulating the expression of macrophage migration inhibitory factor (MIF) and Kruppel-like factor (KLF) 2 gene while activating AMP-activated protein kinase (AMPK). UVA-induced inhibition of adipogenic differentiation was mediated by reducing MIF production through suppression of NF-kappa B. Taken together, these findings suggest that sinapic acid may ameliorate UVA-irradiation-induced reduced stemness and differentiation potential of hAMSCs. Therefore, sinapic acid might have potential as an antagonist agent to attenuate damages caused by UVA.

引用

页数：12

共 42 条

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