Addressable DNA-Myoglobin Photocatalysis

被引：17

作者：

Kuo, Chi-Hsien ^{[1
]}

Fruk, Ljiljana ^{[1
,2
]}

Niemeyer, Christof M. ^{[1
]}

机构：

[1] Tech Univ Dortmund, Fak Chem Biol Chem Mikrostrukturtech, D-44227 Dortmund, Germany

[2] Univ Karlsruhe, Ctr Funct Nanostruct, D-76131 Karlsruhe, Germany

来源：

CHEMISTRY-AN ASIAN JOURNAL | 2009年 / 4卷 / 07期

关键词：

bioorganic chemistry; DNA; photochemistry; protein conjugates; redox chemistry; ELECTRON-TRANSFER; SEMISYNTHETIC PHOTOENZYME; RECONSTITUTED MYOGLOBIN; HEME-RECONSTITUTION; PROTEIN; DESIGN; COFACTOR; MUTATION; ENZYMES; METALLOPROTEINS;

D O I：

10.1002/asia.200900082

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Photoactivatable myoglobin containing a DNA oligonucleotide as a structural anchor was designed by using the reconstitution of artificial heme moieties containing Ru3+ ions. This semisynthetic DNA-enzyme conjugate was successfully used for the oxidation of peroxidase substrates by using visible light instead of H2O2 for the activation. The DNA anchor was utilized for the immobilization of the enzyme oil the surface of magnetic microbeads. Enzyme activity measurements not only indicated undisturbed biofunctionality of the tethered DNA but also enabled magnetic separation-based enrichment and recycling of the photoactivatable biocatalyst.

引用

页码：1064 / 1069

页数：6

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