Enzymatically Cross-Linked Gelatin/Chitosan Hydrogels: Tuning Gel Properties and Cellular Response

被引：39

作者：

da Silva, Marcelo A. ^{[1
]}

Bode, Franziska ^{[1
]}

Drake, Alex F. ^{[2
]}

Goldoni, Silvia ^{[3
,4
]}

Stevens, Molly M. ^{[3
,4
]}

Dreiss, Cecile A. ^{[1
]}

机构：

[1] Kings Coll London, Inst Pharmaceut Sci, London SE1 9NH, England

[2] Kings Coll London, Biomol Spect Ctr, London SE1 1UL, England

[3] Univ London Imperial Coll Sci Technol & Med, Dept Bioengn, Dept Mat, London SW7 2AZ, England

[4] Univ London Imperial Coll Sci Technol & Med, Inst Biomed Engn, London SW7 2AZ, England

来源：

MACROMOLECULAR BIOSCIENCE | 2014年 / 14卷 / 06期

基金：

英国工程与自然科学研究理事会;

关键词：

chitosan; enzymatic cross-linking; hydrogels; rheology; tilapia fish gelatin; VISCOELASTIC PROPERTIES; CHEMICAL NETWORKS; GELATIN NETWORKS; CHITOSAN; TRANSGLUTAMINASE; CONFORMATIONS; BIOMATERIALS; STABILITY; PEPTIDES; COLLAGEN;

D O I：

10.1002/mabi.201300472

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This work investigates the effect of combining physical and chemical gelation processes in a biopolymer blend: chitosan and tilapia fish gelatin. Chemical (C) gels are obtained by cross-linking with the microbial enzyme transglutaminase at 37 degrees C. Hybrid physical-co-chemical (PC) gels are cross-linked at 21 degrees C, below gelatin gelation temperature. These protocols provide two microenvironments for the gelation process: in C gels, both gelatin and chitosan are present as single strands; in PC gels, cross-linking proceeds within a transient physical gel of gelatin, filled by chitosan strands. The chitosan/gelatin chemical networks generated in PC gels show a consistently higher shear modulus than pure C gels; they are also less turbid than their C gels counterparts, suggesting a more homogeneous network. Finally, chitosan enhances the gels' shear modulus in all gels. Proliferation assays show that MC3T3 cells proliferate in these mixed, hybrid gels and better so on PC gels than in C mixed gels.

引用

页码：817 / 830

页数：14

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