Serum untargeted lipidomic profiling reveals dysfunction of phospholipid metabolism in subclinical coronary artery disease

被引：39

作者：

Djekic, Demir ^{[1
]}

Pinto, Rui ^{[2
]}

Repsilber, Dirk ^{[3
]}

Hyotylainen, Tuulia ^{[4
]}

Henein, Michael ^{[5
,6
,7
]}

机构：

[1] Orebro Univ, Sch Med Sci, Dept Cardiol, S-70185 Orebro, Sweden

[2] Imperial Coll London, Fac Med, Sch Publ Hlth, Dept Epidemiol & Biostat, London, England

[3] Orebro Univ, Fac Med & Hlth, Sch Med Sci, Orebro, Sweden

[4] Orebro Univ, Man Technol Environm Res Ctr, Sch Sci & Technol, Orebro, Sweden

[5] Umea Univ & Heart Ctr, Dept Publ Hlth & Clin Med, Umea, Sweden

[6] St George Univ, Mol & Clin Res Inst, London, England

[7] Brunel Univ, Inst Environm Hlth & Phys Sci, London, England

来源：

VASCULAR HEALTH AND RISK MANAGEMENT | 2019年 / 15卷

关键词：

coronary artery calcification; coronary artery calcium score; lipidomics; triacylglycerol; lipids; atherosclerosis; autophagy; ULTRAFAST COMPUTED-TOMOGRAPHY; CARDIOVASCULAR-DISEASE; HEART-DISEASE; RISK-FACTORS; INSULIN-RESISTANCE; ATHEROSCLEROSIS; TRIGLYCERIDES; A(2); CALCIFICATION; ASSOCIATION;

D O I：

10.2147/VHRM.S202344

中图分类号：

R6 [外科学];

学科分类号：

1002 ; 100210 ;

摘要：

Purpose: Disturbed metabolism of cholesterol and triacylglycerols (TGs) carries increased risk for coronary artery calcification (CAC). However, the exact relationship between individual lipid species and CAC remains unclear. The aim of this study was to identify disturbances in lipid profiles involved in the calcification process, in an attempt to propose potential biomarker candidates. Patients and methods: We studied 70 patients at intermediate risk for coronary artery disease who had undergone coronary calcification assessment using computed tomography and Agatston coronary artery calcium score (CACS). Patients were divided into three groups: with no coronary calcification (NCC; CACS: 0; n=26), mild coronary calcification (MCC; CACS: 1-250; n=27), or severe coronary calcification (SCC; CACS: >250; n=17). Patients' serum samples were analyzed using liquid chromatography-mass spectrometry in an untargeted lipidomics approach. Results: We identified 103 lipids within the glycerolipid, glycerophospholipid, sphingolipid, and sterol lipid classes. After false discovery rate correction, phosphatidylcholine (PC)(16:0/20:4) in higher levels and PC(18:2/18:2), PC(36:3), and phosphatidylethanolamine(20:0/18:2) in lower levels were identified as correlates with SCC compared to NCC. There were no significant differences in the levels of individual TGs between the three groups; however, clustering the lipid profiles showed a trend for higher levels of saturated and monounsaturated TGs in SCC compared to NCC. There was also a trend for lower TG (49:2), TG(51:1), TG(54:5), and TG(56:8) levels in SCC compared to MCC. Conclusion: In this study we investigated the lipidome of patients with coronary calcification. Our results suggest that the calcification process may be associated with dysfunction in autophagy. The lipidomic biomarkers revealed in this study may aid in better assessment of patients with subclinical coronary artery disease.

引用

页码：123 / 135

页数：13

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