In vitro anti-HIV-1 activity of fucoidan from Sargassum swartzii

被引：70

作者：

Dinesh, Subramaniam ^{[1
]}

Menon, Thangam ^{[1
]}

Hanna, Luke E. ^{[2
]}

Suresh, V. ^{[3
]}

Sathuvan, M. ^{[4
]}

Manikannan, M. ^{[5
]}

机构：

[1] Univ Madras, Dept Microbiol, Dr ALM PG IBMS, Madras, Tamil Nadu, India

[2] NIRT, Dept Clin Res, Div HIV AIDS, Madras, Tamil Nadu, India

[3] Annamalai Univ, Dept Bot, Annamalainagar 608002, Tamil Nadu, India

[4] Univ Madras, Ctr Adv Studies Bot, Madras 600025, Tamil Nadu, India

[5] Sathyabama Univ, Ctr Drug Discovery & Dev, Madras 600119, Tamil Nadu, India

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2016年 / 82卷

关键词：

HIV-1; Reverse transcriptase; Fucoidan; Sargassum swartzii; ANTI-HIV ACTIVITY; SULFATED POLYSACCHARIDES; BIOLOGICAL-ACTIVITIES; ANTIVIRAL ACTIVITY; FUCANS; ALGAE;

D O I：

10.1016/j.ijbiomac.2015.09.078

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sargassum swartzii, a marine brown algae with wide range of biological properties belongs to the family Sargassaceae. Bioactive fucoidan fractions (CFF, FF1 and FF2) were isolated from S. swartzii and characterized by linear gradient anion-exchange chromatography and FT-IR. The characterized fucoidan fractions contained mainly sugars, sulfate and uronic acid. In the present study, anti-HIV-1 property of the fucoidan fractions was investigated. Fraction FF2 was found to exhibit significant anti-HIV-1 activity at concentrations of 1.56 and 6.25 mu g/ml as observed by >50% reduction in HIV-1 p24 antigen levels and reverse transcriptase activity. Fucoidan fractions have no cytotoxic effects on PBMCs at the concentration range of 1.56-1000 mu g/ml. These results suggest that fucoidan fractions could have inhibitory activity against HIV and has potential as an anti-HIV-1 agent. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：83 / 88

页数：6

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