Identifying Nonspecific Ligand Binding in Electrospray Ionization Mass Spectrometry Using the Reporter Molecule Method

被引：23
作者：
Sun, Nian
Sun, Jiangxiao
Kitova, Elena N.
Klassen, John S. [1 ]
机构：
[1] Univ Alberta, Alberta Ingenu Ctr Carbohydrate Sci, Edmonton, AB T6G 2G2, Canada
来源：
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2009年 / 20卷 / 07期
基金：
加拿大自然科学与工程研究理事会;
关键词：
NANOELECTROSPRAY IONIZATION; ETHIDIUM-BROMIDE; NUCLEIC ACIDS; COMPLEXES; DNA; DISSOCIATION; AFFINITIES; STABILITY; CONSTANTS; DUPLEX;
D O I：
10.1016/j.jasms.2009.02.024
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The application of the reporter molecule (M-rep) method for identifying nonspecific complexes in the ES-MS analysis of protein-ligand and DNA-ligand interactions in vitro is described. To test the reliability of the method, it was applied to the ES-MS analysis of protein-carbohydrate complexes originating from specific interactions in solution and from nonspecific interactions in the ES process. These control experiments confirm the basic assumptions underlying the M-rep method, namely that nonspecific ligand binding is a random process, and that the ES droplet histories for specific and nonspecific complexes are distinct. The application of the M-rep method to the ES-MS analysis of the sequential binding of the ethidium cation, a DNA intercalator, to single and double strand oligodeoxynucleotides is also described, and highlights the general utility of the method. (J Am Soc Mass Spectrorn 2009, 20, 1242-1250) (C) 2009 Published by Elsevier Inc. on behalf of American Society for Mass Spectrometry
引用
收藏
页码：1242 / 1250
页数：9
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