The propagation of a porcine epidemic diarrhea virus in swine cell lines

被引：1

作者：

Kadoi, K ^{[1
]}

Sugioka, H ^{[1
]}

Satoh, T ^{[1
]}

Kadoi, BK ^{[1
]}

机构：

[1] Nihon Univ, Coll Bioresource Sci, Lab Biomed Sci, Fujisawa, Kanagawa 2528510, Japan

来源：

MICROBIOLOGICA | 2002年 / 25卷 / 03期

关键词：

PEDV; CPE; plaque; swine cell lines;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A strain of porcine epidemic diarrhea virus (PEDV), P-5V, utilized as a live virus vaccine in Japan was infected to a swine cell lines, KSEK6 and IB-RS-2 cells. Clear CPE, characterized by cellular destruction, started to appear in the infected cells on 2-3 days post infection (DPI) and affected cells was completely degenerated on 4 DPI. The virus was serially passaged in the cells even without addition of trypsin. Small but clear plaques were formed under an agar overlay medium on the cells. The infective titer in the order of 10(7.00-7.50) TCID50 per ml was obtained at usual incubation temperature.

引用

页码：285 / 290

页数：6

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