DETECTION OF VIRULENCE ASSOCIATED FACTORS FROM STAPHYLOCOCCUS AUREUS ISOLATED FROM BOVINE MASTITIS

被引：0

作者：

Parth, F. M. ^{[1
]}

Chauhan, H. C. ^{[1
]}

Bhagat, A. G. ^{[1
]}

Chandel, B. S. ^{[1
]}

Patel, M. V. ^{[1
]}

Dadawala, A. I. ^{[1
]}

Kher, H. N. ^{[1
]}

机构：

[1] Sardarkrushinagar Dantiwada Agr Univ, Coll Vet Sci & Anim Husb, Dept Vet Microbiol, Palanpur, Gujarat, India

来源：

BUFFALO BULLETIN | 2016年 / 35卷 / 04期

关键词：

Staphylococcus aureus; mastitis; virulence factor; PCR; FRAGMENT-LENGTH-POLYMORPHISM; SUBCLINICAL MASTITIS; SLIME PRODUCTION; GENES; MILK; IDENTIFICATION; RESISTANCE; STRAINS; HUMANS; COWS;

D O I：

暂无

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

A total of 53 bacterial isolates obtained from bovine mastitis and positive to catalase, and 23S rRNA ribotyping tests were analyzed for production virulence factor viz. mannitol fermentation, Coagulase production, lipase activity, DNase activity, slime production assay and haemolysin production. Out of 53 isolates, 53 (100%), 49 (92.45%), 29 (54.72%), 44 (83.02%) and 42 (79.25%) isolates were found positive for mannitol fermentation, Coagulase production, lipase activity, DNase activity and slime production respectively. Out of 53 isolates, alpha, beta, gamma and alpha-beta haemolysin production were observed in 18 (33.96%), 26 (49.06%), 4 (7.55%) and 5 (9.43%) isolates respectively. Further the isolates were subjected to genotypic evaluation of virulence associated Coa and spa genes. Polymorphism was recorded in Coa and spa genes. Amplification of the spa gene revealed 200, 270 and 296 bp size amplicons while 723, 812 and 1000 bp amplicons found in Coa gene amplification. Out of 53 isolates, 36 (67.92%) and 32 (60.38%) isolates were found positive for spa and Coa genes respectively.

引用

页码：697 / 706

页数：10

共 44 条

[11] SPECIES IDENTIFICATION, ANTIBIOTIC-SENSITIVITY AND SLIME PRODUCTION OF COAGULASE-NEGATIVE STAPHYLOCOCCI ISOLATED FROM CLINICAL SPECIMENS [J].