Panel of 23S rRNA Gene-Based Real-Time PCR Assays for Improved Universal and Group-Specific Detection of Phytoplasmas

被引：57

作者：

Hodgetts, Jennifer ^{[1
]}

Boonham, Neil ^{[2
]}

Mumford, Rick ^{[2
]}

Dickinson, Matthew ^{[1
]}

机构：

[1] Univ Nottingham, Sch Biosci, Loughborough LE12 5RD, Leics, England

[2] Cent Sci Lab, York YO41 1LZ, N Yorkshire, England

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2009年 / 75卷 / 09期

关键词：

CANDIDATUS-PHYTOPLASMA; FLAVESCENCE DOREE; PLANTS; IDENTIFICATION; YELLOWS; SEQUENCES; GRAPEVINE; RFLP;

D O I：

10.1128/AEM.02610-08

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Primers and probes based on the 23S rRNA gene have been utilized to design a range of real-time PCR assays for routine phytoplasma diagnostics. These assays have been authenticated as phytoplasma specific and shown to be at least as sensitive as nested PCR. A universal assay to detect all phytoplasmas has been developed, along with a multiplex assay to discriminate 16SrI group phytoplasmas from members of all of the other 16Sr groups. Assays for the 16SrII, 16SrIV, and 16SrXII groups have also been developed to confirm that the 23S rRNA gene can be used to design group-specific assays.

引用

页码：2945 / 2950

页数：6

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