Human Serum Albumin-Gold Nanoparticle Based Impedimetric Sensor for Sensitive Detection of miRNA-200c

Here, human serum albumin conjugated gold nanoparticles (HSA-AuNPs) were synthesized by a simple route to develop an impedimetric sensor for miRNA-200c detection based on a selective oligo-hybridization process without any labeling. The synthetic DNA capture probe for miRNA-200c was decorated onto the HSA-AuNPs modified pencil graphite electrodes. Impedimetric signals were monitored after the hybridization process between the DNA probe and target miRNA-200c. HSA-AuNPs adsorption time, incubation time of the capture probe and hybridization time-temperature were optimized. The proposed miRNA-200c biosensor demonstrated proper sensitivity and selectivity, low detection limit (1.13 fM), good reproducibility and simple direct detection of miRNA-200c in serum.