Brassica villosa, a system for studying non-glandular trichomes and genes in the Brassicas

被引:18
作者
Nayidu, Naghabushana K. [1 ,2 ]
Tan, Yifang [3 ]
Taheri, Ali [1 ]
Li, Xiang [4 ]
Bjorndahl, Trent C. [5 ]
Nowak, Jacek [3 ]
Wishart, David S. [5 ]
Hegedus, Dwayne [1 ]
Gruber, Margaret Y. [1 ]
机构
[1] Agr & Agri Food Canada, Saskatoon Res Ctr, Saskatoon, SK S7N 0X2, Canada
[2] Univ Saskatchewan, Dept Biol, Saskatoon, SK S7N 5E2, Canada
[3] CNR, Saskatoon, SK S7N 0W9, Canada
[4] Jilin Univ, Coll Plant Sci, Changchun 130062, Peoples R China
[5] Univ Alberta, Dept Biol Sci & Comp Sci, Edmonton, AB T6G 1A6, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
Trichome preparations; Metals; Global gene expression; Secondary metabolites; Brassica villosa; Brassica napus; AGROBACTERIUM-MEDIATED TRANSFORMATION; MICROARRAY ANALYSIS; ARABIDOPSIS TRICHOME; CELLULAR COMPARTMENTATION; DNA MICROARRAYS; FATTY-ACID; EXPRESSION; CADMIUM; BIOSYNTHESIS; RESISTANCE;
D O I
10.1007/s11103-014-0201-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Brassica villosa is a wild Brassica C genome species with very dense trichome coverage and strong resistance to many insect pests of Brassica oilseeds and vegetables. Transcriptome analysis of hairy B. villosa leaves indicated higher expression of several important trichome initiation genes compared with glabrous B. napus leaves and consistent with the Arabidopsis model of trichome development. However, transcripts of the TRY inhibitory gene in hairy B. villosa were surprisingly high relative to B. napus and relative transcript levels of SAD2, EGL3, and several XIX genes were low, suggesting potential ancillary or less important trichome-related roles for these genes in Brassica species compared with Arabidopsis. Several antioxidant, calcium, non-calcium metal and secondary metabolite genes also showed differential expression between these two species. These coincided with accumulation of two alkaloid-like compounds, high levels of calcium, and other metals in B. villosa trichomes that are correlated with the known tolerance of B. villosa to high salt and the calcium-rich natural habitat of this wild species. This first time report on the isolation of large amounts of pure B. villosa trichomes, on trichome content, and on relative gene expression differences in an exceptionally hairy Brassica species compared with a glabrous species opens doors for the scientific community to understand trichome gene function in the Brassicas and highlights the potential of B. villosa as a trichome research platform.
引用
收藏
页码:519 / 539
页数:21
相关论文
共 102 条
[1]   Ecological genetics of an induced plant defense against herbivores: Additive genetic variance and costs of phenotypic plasticity [J].
Agrawal, AA ;
Conner, JK ;
Johnson, MTJ ;
Wallsgrove, R .
EVOLUTION, 2002, 56 (11) :2206-2213
[2]   EVOLUTION OF TRICHOME NUMBER IN A NATURALIZED POPULATION OF BRASSICA-RAPA [J].
AGREN, J ;
SCHEMSKE, DW .
AMERICAN NATURALIST, 1994, 143 (01) :1-13
[3]   THE COST OF DEFENSE AGAINST HERBIVORES - AN EXPERIMENTAL-STUDY OF TRICHOME PRODUCTION IN BRASSICA-RAPA [J].
AGREN, J ;
SCHEMSKE, DW .
AMERICAN NATURALIST, 1993, 141 (02) :338-350
[4]   ARTIFICIAL SELECTION ON TRICHOME NUMBER IN BRASSICA-RAPA [J].
AGREN, J ;
SCHEMSKE, DW .
THEORETICAL AND APPLIED GENETICS, 1992, 83 (6-7) :673-678
[5]   Transcriptome analysis of alfalfa glandular trichomes [J].
Aziz, N ;
Paiva, NL ;
May, GD ;
Dixon, RA .
PLANTA, 2005, 221 (01) :28-38
[6]   TRICHOME PATTERNING IN ARABIDOPSIS THALIANA: FROM GENETIC TO MOLECULAR MODELS [J].
Balkunde, Rachappa ;
Pesch, Martina ;
Huelskamp, Martin .
PLANT DEVELOPMENT, 2010, 91 :299-321
[7]   MLEV-17-BASED TWO-DIMENSIONAL HOMONUCLEAR MAGNETIZATION TRANSFER SPECTROSCOPY [J].
BAX, A ;
DAVIS, DG .
JOURNAL OF MAGNETIC RESONANCE, 1985, 65 (02) :355-360
[8]   CORRELATION OF PROTON AND N-15 CHEMICAL-SHIFTS BY MULTIPLE QUANTUM NMR [J].
BAX, A ;
GRIFFEY, RH ;
HAWKINS, BL .
JOURNAL OF MAGNETIC RESONANCE, 1983, 55 (02) :301-315
[9]   H-1 AND C-13 ASSIGNMENTS FROM SENSITIVITY-ENHANCED DETECTION OF HETERONUCLEAR MULTIPLE-BOND CONNECTIVITY BY 2D MULTIPLE QUANTUM NMR [J].
BAX, A ;
SUMMERS, MF .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1986, 108 (08) :2093-2094
[10]  
Bax A, 1986, J MAGN RESON, V86, P346