Reassessment of the Broth Microdilution Method for Susceptibility Testing of Helicobacter pylori

被引：13
作者：
Tang, Xiaoqiong [1 ,2 ,3 ]
Shen, Yalin [1 ,2 ,3 ]
Song, Xiaona [1 ,2 ,3 ]
Benghezal, Mohammed [1 ,2 ,3 ]
Marshall, Barry J. [1 ,2 ,3 ,4 ,5 ]
Tang, Hong [1 ,2 ,3 ]
Li, Hong [1 ,2 ,3 ]
机构：
[1] Sichuan Univ, West China Hosp, West China Marshall Res Ctr Infect Dis, Ctr Infect Dis, Chengdu, Peoples R China
[2] Sichuan Univ, West China Hosp, Div Infect Dis, State Key Lab Biotherapy, Chengdu, Peoples R China
[3] Sichuan Univ, West China Hosp, Ctr Infect Dis, Chengdu, Peoples R China
[4] Univ Western Australia, Marshall Ctr Infect Dis Res & Training, Sch Biomed Sci, Helicobacter pylori Res Lab, Nedlands, Australia
[5] Shenzhen Univ, Sch Biomed Engn, Marshall Lab Biomed Engn, Hlth Sci Ctr, Shenzhen, Peoples R China
来源：
JOURNAL OF INFECTIOUS DISEASES | 2022年 / 226卷
基金：
中国国家自然科学基金;
关键词：
agar dilution; antibiotic susceptibility testing; broth microdilution; helicobacter pylori; DISK DIFFUSION; AGAR DILUTION; BISMUTH; CLARITHROMYCIN; METRONIDAZOLE; CONSENSUS; THERAPY;
D O I：
10.1093/infdis/jiac389
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Background Helicobacter pylori infection is an infectious disease and thus the eradication treatment should be guided by susceptibility testing. This study aimed to assess the applicability of broth microdilution as a routine susceptibility testing method for H. pylori. Methods Susceptibility profiles of clarithromycin (CLR) and levofloxacin (LEV) resistance in 76 clinical H. pylori isolates were simultaneously assessed using agar dilution and broth microdilution methods. The correlation between the minimum inhibitory concentrations (MICs) obtained by the 2 methods was assessed by means of linear regression analysis. Results The correlation between the MICs determined by broth microdilution method and agar dilution method was good for both CLR (r = 0.966) and LEV (r = 0.959). The susceptibility agreement between the 2 methods was 100% for CLR and 96.1% for LEV. Using the broth microdilution method, the false resistance was found in 3.9% (3 of 76) strains for LEV susceptibility testing. No false susceptibility was found for either CLR or LEV, and no false resistance was found for susceptibility testing of CLR. Conclusions The broth microdilution method is suitable for routine susceptibility testing of clinical H. pylori isolates.
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收藏
页码：S486 / S492
页数：7
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