Comparison of the osteogenic potential of mesenchymal stem cells from the bone marrow and adipose tissue of young dogs

被引：22
作者：
Alves, Endrigo G. L. [1 ,2 ]
Serakides, Rogeria [2 ]
Boeloni, Jankerle N. [2 ]
Rosado, Isabel R. [2 ]
Ocarino, Natalia M. [2 ]
Oliveira, Humberto P. [2 ]
Goes, Alfredo M. [3 ]
Rezende, Cleuza M. F. [2 ]
机构：
[1] Univ Uberaba UNIUBE, Curso Med Vet, Uberaba, Brazil
[2] Univ Fed Minas Gerais, Escola Vet, NCT TCA Dept Clin & Cirurgia, Belo Horizonte, MG, Brazil
[3] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Immunol, Belo Horizonte, MG, Brazil
来源：
BMC VETERINARY RESEARCH | 2014年 / 10卷
关键词：
Mesenchymal stem cells; Osteogenic differentiation; Dogs; PHYSICAL-ACTIVITY; STROMAL CELLS; DIFFERENTIATION; CANINE; RATS;
D O I：
10.1186/s12917-014-0190-y
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Background: The aim of the present study was to compare the osteogenic potential of mesenchymal stem cells extracted from the bone marrow (BM-MSCs) and adipose tissue (AD-MSCs) of young dogs. The following parameters were assessed: dimethyl thiazolyl diphenyl tetrazolium (MTT) conversion, alkaline phosphatase (ALP) activity, collagen and mineralised matrix synthesis, and the expressions of osterix, bone sialoprotein (BSP), and osteocalcin (OC). Results: MTT conversion was greater in BM-MSCs compared to AD-MSCs after 14 and 21 days of differentiation; ALP activity was greater in differentiated AD-MSCs on day 7; collagen synthesis was greater in BM-MSCs on days 14 and 21; the percentage of mineralized area per field was greater in BM-MSCs compared to AD-MSCs; osterix expression was greater in BM-MSCs in days 14 and 21, and BSP and OC expression levels were greater in BM-MSCs at all the investigation time-points. Conclusions: It was concluded that the osteogenic potential was greater in BM-MSCs than AD-MSCs when extracted from young dogs.
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页数：9
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