Untargeted LC-Q-TOF mass spectrometry method for the detection of adulterations in skimmed-milk powder

被引：37

作者：

Cordewener, Jan H. G. ^{[1
]}

Luykx, Dion M. A. M. ^{[2
]}

Frankhuizen, Rob ^{[2
]}

Bremer, Maria G. E. G. ^{[2
]}

Hooijerink, Hendricus ^{[2
]}

America, Antoine H. P. ^{[1
]}

机构：

[1] PRI, NL-6708 PB Wageningen, Netherlands

[2] RIKILT, Inst Food Safety, Wageningen Ur, Netherlands

来源：

JOURNAL OF SEPARATION SCIENCE | 2009年 / 32卷 / 08期

关键词：

Adulteration; Comparative LC-MS; Milk powder; Plant proteins; Q-TOF; QUANTITATIVE PROTEOMIC ANALYSIS; DAIRY-PRODUCTS; FOOD PROTEINS; IDENTIFICATION; MS;

D O I：

10.1002/jssc.200800568

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A nontargeted protein identification method was developed to screen for adulterations in skimmed-milk powder (SNIP). There are indications of falsified SNIP content due to the addition of plant proteins. To demonstrate the reliability and accuracy of the developed comparative LC-MS method using a quadrupole TOF MS instrument, adulterated SMP samples were prepared by the addition of protein isolates of soy and pea to skimmed-milk before pasteurisation and evaporation. The comparative LGMS approach enabled unequivocal discrimination of those SNIP samples containing soy and pea protein from nonadulterated SNIP. To identify the source of (plant) proteins present in the adulterated SNIP, data-dependent LC-MS/MS was used in combination with an include list of differential peptides. Numerous peptides originating from the major seed proteins of soy (glycinin, beta-conglycin) and pea (legumin, vicilin) could be identified in this way.

引用

页码：1216 / 1223

页数：8

共 23 条

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