Metabolic regulation of selective deuterium incorporation into RNA and protein components of the ribosome

被引：0

作者：

Shcherbakova, IV ^{[1
]}

Zgurskaya, EI ^{[1
]}

Fan, LX ^{[1
]}

Serdyuk, IN ^{[1
]}

Zaccai, G ^{[1
]}

机构：

[1] RUSSIAN ACAD SCI,INST PROT RES,PUSHCHINO 142292,MOSCOW REGION,RUSSIA

来源：

BIOLOGICAL MACROMOLECULAR DYNAMICS | 1997年

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Selective C-14 or deuterium incorporation into the RNA and protein components of the ribosome during cell growth was monitored by radioactivity measurements and H-1-NMR as a function of C-14-nucleoside concentration and medium D2O percentage in the presence of H-or D- succinate and glycerol. It was demonstrated that in concentrations of nucleosides up to 1.2 mM, nucleoside is essentially incorporated directly into RNA with negligible cross-over into protein as well as negligible incorporation into RNA of deuterium from either the succinate, glycerol substrates or the water.

引用

页码：165 / 169

页数：5