A Comprehensive Subcellular Atlas of the Toxoplasma Proteome via hyperLOPIT Provides Spatial Context for Protein Functions

被引：188

作者：

Barylyuk, Konstantin ^{[1
]}

Koreny, Ludek ^{[1
]}

Ke, Huiling ^{[1
]}

Butterworth, Simon ^{[1
]}

Crook, Oliver M. ^{[1
,2
,3
]}

Lassadi, Imen ^{[1
]}

Gupta, Vipul ^{[1
]}

Tromer, Eelco ^{[1
]}

Mourier, Tobias ^{[4
]}

Stevens, Tim J. ^{[5
]}

Breckels, Lisa M. ^{[1
,2
]}

Pain, Arnab ^{[4
,6
,7
]}

Lilley, Kathryn S. ^{[1
,2
]}

Waller, Ross F. ^{[1
]}

机构：

[1] Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England

[2] Univ Cambridge, Jeffrey Cheah Biomed Ctr, Milner Therapeut Inst, Cambridge CB20 0AW, England

[3] Cambridge Inst Publ Hlth, MRC Biostat Unit, Cambridge CB2 0SR, England

[4] King Abdullah Univ Sci & Technol, Biol & Environm Sci & Engn Div, Thuwal 23955, Saudi Arabia

[5] MRC Lab Mol Biol, Cambridge CB2 0QH, England

[6] Hokkaido Univ, Gi CoRE, Global Stn Zoonosis Control, Sapporo, Hokkaido 0600808, Japan

[7] Univ Oxford, Nuffield Div Clin Lab Sci NDCLS, Oxford OX3 9DU, England

来源：

CELL HOST & MICROBE | 2020年 / 28卷 / 05期

基金：

英国惠康基金; 英国医学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

apicomplexa; evolution; host-pathogen interaction; invasion; organelle; parasitism; plasmodium; proteomics; subcellular; toxoplasma;

D O I：

10.1016/j.chom.2020.09.011

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Apicomplexan parasites cause major human disease and food insecurity. They owe their considerable success to highly specialized cell compartments and structures. These adaptations drive their recognition, nondestructive penetration, and elaborate reengineering of the host's cells to promote their growth, dissemination, and the countering of host defenses. The evolution of unique apicomplexan cellular compartments is concomitant with vast proteomic novelty. Consequently, half of apicomplexan proteins are unique and uncharacterized. Here, we determine the steady-state subcellular location of thousands of proteins simultaneously within the globally prevalent apicomplexan parasite Toxoplasma gondii. This provides unprecedented comprehensive molecular definition of these unicellular eukaryotes and their specialized compartments, and these data reveal the spatial organizations of protein expression and function, adaptation to hosts, and the underlying evolutionary trajectories of these pathogens.

引用

页码：752 / +

页数：24

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