Hybridization chain reactions on silica coated Qbeads for the colorimetric detection of multiplex microRNAs

被引:46
作者
Guo, Qingsheng [1 ]
Bian, Feika [1 ]
Liu, Yuqian [1 ]
Qu, Xiaojun [1 ]
Hu, Xianyun [1 ]
Sun, Qingjiang [1 ]
机构
[1] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China
关键词
FREE SIGNAL AMPLIFICATION; SENSITIVE DETECTION; LIVING CELLS; DNA; CANCER; ASSAY; EXPRESSION; NETWORKS; PROBES;
D O I
10.1039/c7cc00462a
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A robust Qbead platform that integrated with target-binding, hybridization chain reaction and staining was developed for the direct multiplexed detection of endogenous miRNAs by amplified Qbead-colour change.
引用
收藏
页码:4954 / 4957
页数:4
相关论文
共 33 条
  • [1] Scanometric MicroRNA Array Profiling of Prostate Cancer Markers Using Spherical Nucleic Acid-Gold Nanoparticle Conjugates
    Alhasan, Ali H.
    Kim, Dae Y.
    Daniel, Weston L.
    Watson, Erin
    Meeks, Joshua J.
    Thaxton, C. Shad
    Mirkin, Chad A.
    [J]. ANALYTICAL CHEMISTRY, 2012, 84 (09) : 4153 - 4160
  • [2] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004)
    Bartel, David P.
    [J]. CELL, 2007, 131 (04) : 11 - 29
  • [3] MicroRNA expression profiling using LNA-modified probes in a liquid-phase bead-based array
    Busch, Anna Karina
    Litman, Thomas
    Nielsen, Peter Stein
    [J]. NATURE METHODS, 2007, 4 (05) : I - ii
  • [4] Campuzano S., 2014, ANGEW CHEM, V126, P6282
  • [5] Supramolecular Spectrally Encoded Microgels with Double Strand Probes for Absolute and Direct miRNA Fluorescence Detection at High Sensitivity
    Causa, Filippo
    Aliberti, Anna
    Cusano, Angela M.
    Battista, Edmondo
    Netti, Paolo A.
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2015, 137 (05) : 1758 - 1761
  • [6] Highly sensitive and specific microRNA expression profiling using BeadArray technology
    Chen, Jing
    Lozach, Jean
    Wickham, Eliza
    Barnes, Garcia Bret
    Luo, Shujun
    Mikoulitch, Ivan
    Zhou, Lixin
    Schroth, Gary
    Fan, Jian-Bing
    [J]. NUCLEIC ACIDS RESEARCH, 2008, 36 (14)
  • [7] Triggered amplification by hybridization chain reaction
    Dirks, RM
    Pierce, NA
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (43) : 15275 - 15278
  • [8] MicroRNA: Function, Detection, and Bioanalysis
    Dong, Haifeng
    Lei, Jianping
    Ding, Lin
    Wen, Yongqiang
    Ju, Huangxian
    Zhang, Xueji
    [J]. CHEMICAL REVIEWS, 2013, 113 (08) : 6207 - 6233
  • [9] Numerous microRNPs in neuronal cells containing novel microRNAs
    Dostie, J
    Mourelatos, Z
    Yang, M
    Sharma, A
    Dreyfuss, G
    [J]. RNA, 2003, 9 (02) : 180 - 186
  • [10] Oncomirs - microRNAs with a role in cancer
    Esquela-Kerscher, A
    Slack, FJ
    [J]. NATURE REVIEWS CANCER, 2006, 6 (04) : 259 - 269