Production of polyclonal antiserum specific to the 27.5 kDa envelope protein of white spot syndrome virus

被引：20
作者：
You, ZO [1 ]
Nadala, ECB
Yang, JS
van Hulten, MCW
Loh, PC
机构：
[1] Univ Hawaii, Dept Microbiol, Honolulu, HI 96822 USA
[2] Univ Cambridge, Dept Haematol, Cambridge CB2 2PT, England
[3] Univ Wageningen & Res Ctr, Virol Lab, NL-6709 PD Wageningen, Netherlands
来源：
DISEASES OF AQUATIC ORGANISMS | 2002年 / 51卷 / 01期
关键词：
white spot syndrome virus; 27.5 kDa structural protein; rabbit polyclonal antiserum;
D O I：
10.3354/dao051077
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
A truncated version of the white spot syndrome virus (WSSV) 27.5 kDa envelope protein was expressed as a histidine tag fusion protein in Escherichia coli. The bacterial expression system allowed the production of up to 10 mg of purified recombinant protein per liter of bacterial culture. Antiserum from a rabbit immunized with the recombinant protein recognized the 27.5 kDa viral envelope protein of WSSV isolated from different geographic regions. The antiserum did not recognize any of the other known WSSV structural proteins. A sensitive immunodot assay for WSSV was developed using the specific rabbit polyclonal antiserum.
引用
收藏
页码：77 / 80
页数：4
相关论文
共 20 条
  • [1] Chen SN, 1995, SWIMMING THROUGH TROUBLED WATER, P29
  • [2] Pathogenicity of a baculovirus infection causing white spot syndrome in cultured penaeid shrimp in Taiwan
    Chou, HY
    Huang, CY
    Wang, CH
    Chiang, HC
    Lo, CF
    [J]. DISEASES OF AQUATIC ORGANISMS, 1995, 23 (03) : 165 - 173
  • [3] DURAND S, 1996, DIS AQUAT ORGAN, V27, P58
  • [4] Hameed AS, 1998, AQUACULTURE, V160, P31, DOI 10.1016/S0044-8486(97)00221-4
  • [5] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
    LAEMMLI, UK
    [J]. NATURE, 1970, 227 (5259) : 680 - +
  • [6] Risk of spread of penaeid shrimp viruses in the Americas by the international movement of liver and frozen shrimp
    Lightner, DV
    Redman, RM
    Poulos, BT
    Nunan, LM
    Mari, JL
    Hasson, KW
    [J]. REVUE SCIENTIFIQUE ET TECHNIQUE-OFFICE INTERNATIONAL DES EPIZOOTIES, 1997, 16 (01): : 146 - 160
  • [7] White spot syndrome baculovirus (WSBV) detected in cultured and captured shrimp, crabs and other arthropods
    Lo, CF
    Ho, CH
    Peng, SE
    Chen, CH
    Hsu, HC
    Chiu, YL
    Chang, CF
    Liu, KF
    Su, MS
    Wang, CH
    Kou, GH
    [J]. DISEASES OF AQUATIC ORGANISMS, 1996, 27 (03) : 215 - 225
  • [8] Loh PC, 1998, NEW DEVELOPMENTS IN MARINE BIOTECHNOLOGY, P295
  • [9] The pathogenicity of a baculo-like virus isolated from diseased penaeid shrimp obtained from China for cultured penaeid species in Hawaii
    Lu, Y
    Tapay, LM
    Loh, PC
    Gose, RB
    Brock, JA
    [J]. AQUACULTURE INTERNATIONAL, 1997, 5 (03) : 277 - 282
  • [10] White spot syndrome virus (WSSV) in cultured Penaeus monodon in the Philippines
    Magbanua, FO
    Natividad, KT
    Migo, VP
    Alfafara, CG
    de la Peña, FO
    Miranda, RO
    Albaladejo, JD
    Nadala, ECB
    Loh, PC
    Mahilum-Tapay, L
    [J]. DISEASES OF AQUATIC ORGANISMS, 2000, 42 (01) : 77 - 82
12