Adenovirus-mediated gene transfer of B7.1 induces immunological anti-tumor effects in a murine brain tumor

被引：11

作者：

Morioka, J ^{[1
]}

Kajiwara, K ^{[1
]}

Yoshikawa, K ^{[1
]}

Ideguchi, M ^{[1
]}

Uchida, T ^{[1
]}

Ohmoto, Y ^{[1
]}

Suzuki, M ^{[1
]}

机构：

[1] Yamaguchi Univ, Sch Med, Dept Neurosurg, Yamaguchi 7558505, Japan

来源：

JOURNAL OF NEURO-ONCOLOGY | 2002年 / 60卷 / 01期

关键词：

adenoviral vector; B7.1 (CD80); gene transfer; glioma; immune gene therapy;

D O I：

10.1023/A:1020260822669

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The purpose of the present study was to determine if adenovirus-mediated transfection of a syngeneic mouse brain tumor with the gene encoding B7.1 enhances immunogenicity against tumor. Malignant astrocytoma cells were transfected with adenoviral vectors carrying the B7.1 gene (AdB7). Immunocytochemical analysis confirmed the expression of B7.1 in vitro and in vivo. To investigate the effects of B7.1 expression on tumorigenicity of the malignant astrocytoma, mice were implanted intracerebrally with B7.1-transfected glioma cells. There was no significant difference in proliferation between B7.1-transfected cells and controls in vitro. Nevertheless, mice implanted with B7.1-transfected cells survived significantly longer than those in the control groups. Immunocytochemical analysis of the tumors showed that there was infiltration of a number of CD8(+) T-cells and CD25(+) activated T-cells in the brain implanted with B7.1-transfected glioma cells. The results showed the possibility that adenovirus-mediated B7.1 gene transfection to a brain tumor induced activation of CD8(+) cytotoxic T-lymphocytes.

引用

页码：13 / 23

页数：11

共 39 条

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