Menstrum for culture preservation and medium for seed preparation in a tetanus toxin production process containing no animal or dairy products

被引：6

作者：

Fang, A. ^{[1
]}

Gerson, D. F. ^{[1
]}

Demain, A. L. ^{[1
]}

机构：

[1] MIT, Dept Biol, Cambridge, MA USA

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2006年 / 43卷 / 04期

关键词：

Clostridium tetani; culture preservation; seed medium; tetanus toxin;

D O I：

10.1111/j.1472-765X.2006.01984.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aims: To completely eliminate animal and dairy products from the lyophilization menstrum and the seed medium used to produce tetanus toxin with Clostridium tetani. Methods and Results: Tetanus toxin production in a recently developed fermentation medium lacking animal and dairy products was studied with different seed media. It was found that soy peptone could completely replace the beef heart infusion plus animal peptone previously used as seed medium. In addition, we found that cells lyophilized in soy milk could replace the usual type of cells lyophilized in cow's milk. Conclusions: We have now developed a complete tetanus toxin production process containing no animal and dairy products. Significance and Impact of the Study: Toxoid preparations made from toxin produced with animal and dairy products can contain undesirable contaminants such as the prion causing bovine spongiform encephalopathy (Mad Cow's Disease) or antigenic peptides that stimulate anaphylactic reactions and other undesirable immune reactions in immunized hosts. The new vegetable-based process described here avoids such unfortunate possibilities.

引用

页码：360 / 363

页数：4

共 6 条

[1] Effective levels of tetanus toxin can be made in a production medium totally lacking both animal (e.g., brain heart infusion) and dairy proteins or digests (e.g., casein hydrolysates) [J].