Interaction between lysozyme and three flavones by fluorescence spectroscopy

The interaction between lysozyme and three flavones, including Apigenin, Luteocin and Quercetin, were studied by fluorescence spectroscopy. The influence of C3-OH and C3'-OH on the interaction of flavones-Lys were compared. The results showed that C3'-OH could enhance the binding affinity greatly; while C3-OH weakened the affinity largely. According to the structure parameters of assay flavones, the effect of hydrogen group on the flavones-protein were discussed, and the main reason resulting in the weaker binding affinity of Quercetin-Lys were explored. The results obtained from the work could supply beneficial reference for the further study of Flavone-Protein interactions.