Increased fatty acid unsaturation and production of arachidonic acid by homologous over-expression of the mitochondrial malic enzyme in Mortierella alpina

被引：37
作者：
Hao, Guangfei [1 ]
Chen, Haiqin [1 ,2 ]
Du, Kai [1 ]
Huang, Xiaoyun [1 ]
Song, Yuanda [1 ]
Gu, Zhennan [1 ,2 ]
Wang, Lei [3 ]
Zhang, Hao [1 ,2 ]
Chen, Wei [1 ,2 ]
Chen, Yong Q. [1 ,2 ,4 ,5 ]
机构：
[1] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China
[2] Synergist Innovat Ctr Food Safety & Nutr, Wuxi 214122, Peoples R China
[3] Nankai Univ, TEDA Sch Biol Sci & Biotechnol, Tianjin Econ Technol Dev Area, Tianjin 300457, Peoples R China
[4] Wake Forest Sch Med, Dept Canc Biol, Winston Salem, NC 27157 USA
[5] Wake Forest Sch Med, Dept Biochem, Winston Salem, NC 27157 USA
来源：
BIOTECHNOLOGY LETTERS | 2014年 / 36卷 / 09期
基金：
国家高技术研究发展计划(863计划); 中国国家自然科学基金;
关键词：
Arachidonic acid; Fatty acid desaturation; Fatty acid synthesis; Mitochondrial malic enzyme; Mortierella alpina; MUCOR-CIRCINELLOIDES; OLEAGINOUS FUNGUS; MULTIPLE ISOFORMS; MICROORGANISMS; GENES;
D O I：
10.1007/s10529-014-1546-x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Malic enzyme (ME) catalyses the oxidative decarboxylation of l-malate to pyruvate and provides NADPH for intracellular metabolism, such as fatty acid synthesis. Here, the mitochondrial ME (mME) gene from Mortierella alpina was homologously over-expressed. Compared with controls, fungal arachidonic acid (ARA; 20:4 n-6) content increased by 60 % without affecting the total fatty acid content. Our results suggest that enhancing mME activity may be an effective mean to increase industrial production of ARA in M. alpina.
引用
收藏
页码：1827 / 1834
页数：8
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