A perspective on proteomics in cell biology

被引:42
作者
Ahmad, Yasmeen [1 ]
Lamond, Angus I. [1 ]
机构
[1] Univ Dundee, Coll Life Sci, Ctr Gene Regulat & Express, Dundee DD1 5EH, Scotland
关键词
COMPLEX PROTEIN MIXTURES; MASS-SPECTROMETRY; ABSOLUTE QUANTIFICATION; IDENTIFICATION; TANDEM; QUANTITATION; ACCURATE; EXPRESSION; THROUGHPUT; STRATEGY;
D O I
10.1016/j.tcb.2013.10.010
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
During the past 15 years mass spectrometry (MS)-based analyses have become established as the method of choice for direct protein identification and measurement. Owing to the remarkable improvements in the sensitivity and resolution of MS instruments, this technology has revolutionised the opportunities available for the system-wide characterisation of proteins, with wide applications across virtually the whole of cell biology. In this article we provide a perspective on the current state of the art and discuss how the future of cell biology research may benefit from further developments and applications in the field of MS and proteomics, highlighting the major challenges ahead for the community in organising the effective sharing and integration of the resulting data mountain.
引用
收藏
页码:257 / 264
页数:8
相关论文
共 46 条
  • [21] Cell biology and the genome projects - A concerted strategy for characterizing multiprotein complexes by using mass spectrometry
    Lamond, AI
    Mann, M
    [J]. TRENDS IN CELL BIOLOGY, 1997, 7 (04) : 139 - 142
  • [22] Global Subcellular Characterization of Protein Degradation Using Quantitative Proteomics
    Larance, Mark
    Ahmad, Yasmeen
    Kirkwood, Kathryn J.
    Ly, Tony
    Lamond, Angus I.
    [J]. MOLECULAR & CELLULAR PROTEOMICS, 2013, 12 (03) : 638 - 650
  • [23] Correlation of mRNA and protein in complex biological samples
    Maier, Tobias
    Guell, Marc
    Serrano, Luis
    [J]. FEBS LETTERS, 2009, 583 (24): : 3966 - 3973
  • [24] Functional and quantitative proteomics using SILAC
    Mann, Matthias
    [J]. NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2006, 7 (12) : 952 - 958
  • [25] The Coming Age of Complete, Accurate, and Ubiquitous Proteomes
    Mann, Matthias
    Kulak, Nils A.
    Nagaraj, Nagarjuna
    Cox, Juergen
    [J]. MOLECULAR CELL, 2013, 49 (04) : 583 - 590
  • [26] Twenty Years of Gas Phase Structural Biology
    Marcoux, Julien
    Robinson, Carol V.
    [J]. STRUCTURE, 2013, 21 (09) : 1541 - 1550
  • [27] Quantification of the synaptosomal proteome of the rat cerebellum during post-natal development
    McClatchy, Daniel B.
    Liao, Lujian
    Park, Sung Kyu
    Venable, John D.
    Yates, John R.
    [J]. GENOME RESEARCH, 2007, 17 (09) : 1378 - 1388
  • [28] Emerging roles of nuclear protein phosphatases
    Moorhead, Greg B. G.
    Trinkle-Mulcahy, Laura
    Ulke-Lemee, Annegret
    [J]. NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2007, 8 (03) : 234 - 244
  • [29] Accurate quantitation of protein expression and site-specific phosphorylation
    Oda, Y
    Huang, K
    Cross, FR
    Cowburn, D
    Chait, BT
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (12) : 6591 - 6596
  • [30] Megadalton Complexes in the Chloroplast Stroma of Arabidopsis thaliana Characterized by Size Exclusion Chromatography, Mass Spectrometry, and Hierarchical Clustering
    Olinares, Paul Dominic B.
    Ponnala, Lalit
    van Wijk, Klaas J.
    [J]. MOLECULAR & CELLULAR PROTEOMICS, 2010, 9 (07) : 1594 - 1615