Effects of total glucosides of paeony on immune regulatory toll-like receptors TLR2 and 4 in the kidney from diabetic rats

被引：47

作者：

Xu, Xing-xin ^{[1
]}

Qi, Xiang-Ming ^{[1
]}

Zhang, Wei ^{[1
]}

Zhang, Chao-Qun ^{[1
]}

Wu, Xiao-Xu ^{[1
]}

Wu, Yong-Gui ^{[1
]}

Wang, Kun ^{[1
]}

Shen, Ji-Jia ^{[2
]}

机构：

[1] Anhui Med Univ, Affiliated Hosp 1, Dept Nephrol, Hefei, Anhui, Peoples R China

[2] Anhui Med Univ, Dept Pathobiol, Hefei, Anhui, Peoples R China

来源：

PHYTOMEDICINE | 2014年 / 21卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Total glucosides of paeony; Diabetic nephropathy; Inflammation; Toll-like receptors; NF-kappa B; TUMOR-NECROSIS-FACTOR; DENDRITIC CELLS; ACTIVATION; TOLL-LIKE-RECEPTOR-4; PROLIFERATION; EXPRESSION; MECHANISM; KNOCKOUT; INJURY; STATE;

D O I：

10.1016/j.phymed.2013.12.003

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

TLRs are a family of receptors that play a critical role in the pathogenesis of diabetic nephropathy. TGP have been shown to have anti-inflammatory and immuno-regulatory activities. However, the relation between TGP and TLRs on diabetic nephropathy remains unknown. In this study, we examined effects of TGP on immune regulatory TLR2 and 4 in the kidney from streptozotocin-induced diabetic rats. TGP decreased the levels of 24 h urinary albumin excretion rate significantly in diabetic rats. Western blot analysis showed that TGP significantly inhibited the expression of TLR2 and 4, MyD88, p-IRAK1, NF-kappa B p65, p-IRF3, TNF-alpha and IL-1 beta. Quantitative real-time PCR analysis showed that the significantly increased levels of TLR2 and 4, and MyD88mRNA in the kidneys of diabetic rats were significantly suppressed by TGP treatment. Macrophages infiltration were also markedly increased in the kidneys of the diabetic rats, but were significantly inhibited by TGP in a dose-dependent manner. These results suggest that TGP has protective effects on several pharmacological targets in the progress of diabetic nephropathy by selectively blocking TLRs activation in vivo. (C) 2013 Elsevier GmbH. All rights reserved.

引用

页码：815 / 823

页数：9

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