Disposable microfluidic device with ultraviolet detection for highly resolved screening of illicit drugs

被引：21

作者：

Qiang, Wei ^{[1
]}

Zhai, Chun ^{[1
]}

Lei, Jianping ^{[1
]}

Song, Chaojin ^{[2
]}

Zhang, Daming ^{[2
]}

Sheng, Jin ^{[1
]}

Ju, Huangxian ^{[1
]}

机构：

[1] Nanjing Univ, Dept Chem, Minist Educ China, Key Lab Analyt Chem Life Sci, Nanjing 210093, Peoples R China

[2] Beijing Municipal Publ Secur Bur, Beijing 100085, Peoples R China

来源：

ANALYST | 2009年 / 134卷 / 09期

关键词：

ELECTROKINETIC CAPILLARY CHROMATOGRAPHY; SELECTIVE EXHAUSTIVE INJECTION; TANDEM MASS-SPECTROMETRY; OF-CARE DIAGNOSTICS; ORAL FLUID; AMPEROMETRIC DETECTION; EMERGENCY-DEPARTMENT; ZONE ELECTROPHORESIS; ORGANIC MODIFIERS; UV DETECTION;

D O I：

10.1039/b906434f

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A disposable microfluidic device was constructed by conveniently integrating one poly( methyl methacrylate) board with four reservoirs and one fractured fused-silica capillary with 50 mu m i.d. and 7.5 cm total length on a printed circuit board for applying sampling and separation voltages. The disposable microfluidic device combined with a home-made ultraviolet workstation could be conveniently used for efficient screening and quantitative detection of mu g mL(-1) illicit drugs. Using eight illicit drugs as models, they could be baseline-separated within 240 s with the separation efficiency up to 600 047 plates m(-1) at the designed device. The novel device and proposed protocol were successfully used to screen illicit drugs in human urine. This work presented a simple and low-cost method to fabricate the microfluidic device and provided a powerful way for sensitive and specific multi-screening of different drugs with high resolution, fast separation and low-cost.

引用

页码：1834 / 1839

页数：6

共 42 条

[1] Ofloxacin causes false-positive immunoassay results for urine opiates [J].

Backmund, M ;

Meyer, K ;

Von Zielonka, M ;

Eichenlaub, D .

ADDICTION BIOLOGY, 2000, 5 (03) :319-320

[2] Quinolones and false-positive urine screening for opiates by immunoassay technology [J].

Baden, LR ;

Horowitz, G ;

Jacoby, H ;

Eliopoulos, GM .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2001, 286 (24) :3115-3119

[3] On-site testing of illicit drugs:: the use of the drug-testing device "Toxiquick®" [J].

Biermann, T ;

Schwarze, B ;

Zedler, B ;

Betz, R .

FORENSIC SCIENCE INTERNATIONAL, 2004, 143 (01) :21-25

[4] Forensic science [J].

Brettell, T. A. ;

Butler, J. M. ;

Almirall, J. R. .

ANALYTICAL CHEMISTRY, 2007, 79 (12) :4365-4384

[5]

CAMARA PD, 1995, CLIN CHEM, V41, P115

[6] Identification and measurement of illicit drugs and their metabolites in urban wastewater by liquid chromatography-tandem mass spectrometry [J].

Castiglioni, Sara ;

Zuccato, Ettore ;

Crisci, Elisabetta ;

Chiabrando, Chiara ;

Fanelli, Roberto ;

Bagnati, Renzo .

ANALYTICAL CHEMISTRY, 2006, 78 (24) :8421-8429

[7] Underreporting of illicit drug use by patients at emergency departments as revealed by two-tiered urinalysis [J].

Chen, Wei J. ;

Fang, Cheng-Chung ;

Shyu, Ren-Shi ;

Lin, Kuo-Chien .

ADDICTIVE BEHAVIORS, 2006, 31 (12) :2304-2308

[8] Surface patterning of (bio)molecules onto the inner wall of fused-silica capillary tubes [J].

Dendane, Nabil ;

Hoang, Antoine ;

Renaudet, Olivier ;

Vinet, Francoise ;

Dumy, Pascal ;

Defrancq, Eric .

LAB ON A CHIP, 2008, 8 (12) :2161-2163

[9] Widening of the elution window in micellar electrokinetic chromatography with cationic surfactants - I. Selection of surfactant, variation of pH and addition of organic modifiers or inorganic metal salts [J].

Dworschak, A ;

Pyell, U .

JOURNAL OF CHROMATOGRAPHY A, 1999, 848 (1-2) :387-400

[10]

George S, 2002, CLIN CHEM, V48, P1639

← 1 2 3 4 5 →