Development of an efficient enzymatic production of γ-D-glutamyl-L-tryptophan (SCV-07), a prospective medicine for tuberculosis, with bacterial γ-glutamyltranspeptidase

被引:41
作者
Suzuki, H [1 ]
Kato, K
Kumagai, H
机构
[1] Kyoto Univ, Grad Sch Biostudies, Div Integrated Life Sci, Sakyo Ku, Kyoto 6068502, Japan
[2] Kyoto Univ, Fac Agr, Detp Life & Biomat Sci, Sakyo Ku, Kyoto 6068502, Japan
关键词
gamma-glutamyl transpeptidase; gamma-glutamyl transferase; gamma-D-glutamyl-L-tryptophan; SCV-07; stereospecific production; tuberculosis;
D O I
10.1016/j.jbiotec.2004.04.003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
gamma-D-Glutamyl-L-tryptophan (SCV-07) is a prospective medicine for the treatment of tuberculosis, according to the phase two clinical trial. Because gamma-D-glutamyl-L-tryptophan has several reactive groups in its molecule, consists Of D- and L-amino acids, and is connected by gamma-glutamyl linkage, its chemical synthesis is complicated. An efficient enzymatic method to synthesize gamma-D-glutamyl-L-tryptophan from D-glutamine and L-tryptophan employing bacterial gamma-glutamyltranspeptidase was developed. The optimum reaction conditions were 50 mM D-glutamine, 50 mM L-tryptophan, and 0.2 U ml(-1) gamma-glutamyltranspeptidase, pH 9-9.5, and incubation at 37degreesC for 5 h. After a 5 h incubation, 33 mM gamma-D-glutamyl-L-tryptophan was obtained, the conversion rate being 66%. The product was purified by Dowex 1 x 8 column and was considered to be gamma-D-glutamyl-L-tryptophan. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:291 / 295
页数:5
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