Experimental Lactococcus garvieae infection in zebrafish and first evidence of its ability to invade non-phagocytic cells

被引：14

作者：

Aguado-Urda, Monica ^{[1
]}

Rodriguez-Bertos, Antonio ^{[2
,3
]}

de las Heras, Ana I. ^{[4
,5
]}

Blanco, Maria M. ^{[1
]}

Acosta, Felix

Cid, Raquel ^{[5
]}

Fernandez-Garayzabal, Jose F. ^{[1
,3
]}

Gibello, Alicia ^{[1
]}

机构：

[1] Univ Complutense, Fac Vet Sci, Dept Anim Hlth, E-28040 Madrid, Spain

[2] Univ Complutense, Fac Vet Sci, Dept Internal Med & Anim Surg, E-28040 Madrid, Spain

[3] Univ Complutense Madrid, Anim Hlth Surveillance Ctr VISAVET, E-28040 Madrid, Spain

[4] Bioorgan Res & Serv SA, Jerez de la Fontera 11591, Spain

[5] Univ Las Palmas Gran Canaria, Inst Anim Hlth IUSA, Arucas 35413, Spain

来源：

VETERINARY MICROBIOLOGY | 2014年 / 171卷 / 1-2期

关键词：

Lactococcus garvieae; Zebrafish; Experimental infection; Invasion; Non-phagocytic cells; STREPTOCOCCUS-INIAE; IN-VITRO; FISH; PATHOGENICITY; YELLOWTAIL; ADHERENCE; INVASION; MODEL;

D O I：

10.1016/j.vetmic.2014.03.029

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Zebrafish has been used for studying infections and host pathogen interactions in different bacterial fish pathogens. In the present study we evaluated the ability of Lactococcus garvieae to infect zebrafish when inoculated intraperitoneally with 2 x 10(7) UFC of this pathogen. L. garvieae can colonize and invade zebrafish at multiple anatomical sites causing a lethal acute septicemic infection with clinical signs and lesions consistent with those observed in lactococcosis outbreaks. Immunohistochemical studies showed the presence of L garvieae into macrophages as well as into non-phagocytic zebrafish cells of liver (hepatocytes). The internalization capacity, showed by L garvieae in zebrafish cells was confirmed in the rainbow trout cell line RTG-2. Our results provide the first evidence that L garvieae is able to invade non-phagocytic host cells. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：248 / 254

页数：7

共 21 条

[1]

Acosta F, 2002, J AQUAT ANIM HEALTH, V14, P77, DOI 10.1577/1548-8667(2002)014<0077:EOIAMM>2.0.CO

[2]

[3] Invasion and survival of Photobacterium damselae subsp piscicida in non-phagocytic cells of gilthead sea bream, Sparus aurata L. [J].

Acosta, F. ;

Vivas, J. ;

Padilla, D. ;

Vega, J. ;

Bravo, J. ;

Grasso, V. ;

Real, F. .

JOURNAL OF FISH DISEASES, 2009, 32 (06) :535-541

[4] Trolling for the ideal model host: zebrafish take the bait [J].

Allen, Jonathan P. ;

Neely, Melody N. .

FUTURE MICROBIOLOGY, 2010, 5 (04) :563-569

[5]

Avci H., 2013, J FISH DIS

[6] Canine Gastric Carcinoma: Immunohistochemical Expression of Cell Cycle Proteins (p53, p21, and p16) and Heat Shock Proteins (Hsp27 and Hsp70) [J].

Carrasco, V. ;

Canfran, S. ;

Rodriguez-Franco, F. ;

Benito, A. ;

Sainz, A. ;

Rodriguez-Bertos, A. .

VETERINARY PATHOLOGY, 2011, 48 (01) :322-329

[7] Primary infective spondylodiscitis caused by Lactococcus garvieae and a review of human L-garvieae infections [J].

Chan, J. F. W. ;

Woo, P. C. Y. ;

Teng, J. L. L. ;

Lau, S. K. P. ;

Leung, S. S. M. ;

Tam, F. C. C. ;

Yuen, K. -Y. .

INFECTION, 2011, 39 (03) :259-264

[8] In Vitro Study of Adherence, Invasion, and Persistence of Streptococcus iniae in Fibroblastic-Like Fish Cell Line SAF-1 [J].

El Aamri, F. ;

Real, F. ;

Acosta, F. ;

Acosta, B. ;

Valdivia, J. ;

Ramos-Vivas, J. ;

Padilla, D. .

JOURNAL OF AQUATIC ANIMAL HEALTH, 2012, 24 (03) :165-170

[9] Transcytosis of Streptococcus iniae through skin epithelial barriers:: an in vitro study [J].

Eyngor, Marina ;

Chilmonczyk, Stefan ;

Zlotkin, Amir ;

Manuali, Elisabetta ;

Lahav, Dan ;

Ghittino, Claudio ;

Shapira, Roni ;

Hurvitz, Avshalom ;

Eldar, Avi .

FEMS MICROBIOLOGY LETTERS, 2007, 277 (02) :238-248

[10]

Kang Sung Hyun, 2004, J Vet Sci, V5, P387

← 1 2 3 →