Nesfatin-1/nucleobindin-2 like immunoreactivity in the olfactory system, brain and pituitary of frog, Microhyla ornata

被引：14
作者：
Senejani, A. G. [1 ]
Gaupale, Tekchand C. [1 ]
Unniappan, Suraj [2 ]
Bhargava, Shobha [1 ]
机构：
[1] Univ Pune, Dept Zool, Pune 411007, Maharashtra, India
[2] Univ Saskatchewan, Dept Vet Biomed Sci, Lab Integrat Neuroendocrinol, Saskatoon, SK S7N 5B4, Canada
来源：
GENERAL AND COMPARATIVE ENDOCRINOLOGY | 2014年 / 202卷
基金：
加拿大自然科学与工程研究理事会;
关键词：
Nesfatin-1; Amphibians; Brain; Pituitary; Immunohistochemistry; Western blot; MESSENGER-RNA EXPRESSION; NESFATIN-1; HORMONE; LOCALIZATION; IDENTIFICATION; NEURONS;
D O I：
10.1016/j.ygcen.2014.04.007
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Nesfatin-1 is a recently discovered anorectic protein derived from the precursor nucleobindin-2 (NUCB2). While nesfatin-1 has been widely studied in mammals, and goldfish, there are no reports of nesfatin-1 in amphibians. Using immunohistochemistry and Western blot analysis, this study assessed the distribution of NUCB2/nesfatin-1 in the brain of frog Microhyla ornata. NUCB2/nesfatin-1 like immunoreactivity was found in the olfactory receptor neurons, Bowman's glands and in the olfactory epithelium of medial diverticulum. In the brain, immunoreactive perikarya were seen in the anterior preoptic area, magnocellular nucleus, suprachiasmatic nucleus, ventromedial thalamic nucleus, posterior thalamic nucleus, nucleus infundibularis ventralis and dorsalis, and the cerebellar nucleus. NUCB2/nesfatin-1like immunoreactivity was also detected in the pineal and pituitary glands of frog. The presence of NUCB2/nesfatin-1 in the key brain regions suggest possible roles for this protein in the regulation of physiological processes in frogs. (C) 2014 Elsevier Inc. All rights reserved.
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页码：8 / 14
页数：7
相关论文
共 30 条
[21]   Role of the arcuate nucleus of the hypothalamus in regulation of body weight during energy deficit [J].
Sainsbury, A. ;
Zhang, L. .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2010, 316 (02) :109-119
[22]   Nesfatin-1: An Overview and Future Clinical Application [J].
Shimizu, Hiroyuki ;
Oh-I, Sinsuke ;
Okada, Shuichi ;
Mori, Masatomo .
ENDOCRINE JOURNAL, 2009, 56 (04) :537-543
[23]   Identification and Characterization of Nesfatin-1 Immunoreactivity in Endocrine Cell Types of the Rat Gastric Oxyntic Mucosa [J].
Stengel, Andreas ;
Goebel, Miriam ;
Yakubov, Iskandar ;
Wang, Lixin ;
Witcher, Derrick ;
Coskun, Tamer ;
Tache, Yvette ;
Sachs, George ;
Lambrecht, Nils W. G. .
ENDOCRINOLOGY, 2009, 150 (01) :232-238
[24]   The novel function of nesfatin-1: Anti-hyperglycemia [J].
Su, Yijing ;
Zhang, Jing ;
Tang, Yanchun ;
Bi, Feng ;
Liu, Jian-Ning .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2010, 391 (01) :1039-1042
[25]   A STEREOTAXIC ATLAS FOR DIENCEPHALIC NUCLEI OF THE FROG, RANA-PIPIENS [J].
WADA, M ;
URANO, A ;
GORBMAN, A .
ARCHIVUM HISTOLOGICUM JAPONICUM, 1980, 43 (02) :157-173
[26]   Feeding inhibition by urocortin in the rat hypothalamic paraventricular nucleus [J].
Wang, CF ;
Mullet, MA ;
Glass, MJ ;
Billington, CJ ;
Levine, AS ;
Kotz, CM .
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 2001, 280 (02) :R473-R480
[27]   Chromogranin A-immunoreactive cells in the olfactory system of anuran amphibians [J].
Wittle, LW ;
Opalek, JM ;
Ruiter, TC .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 2000, 120 (01) :17-26
[28]   Molecular cloning, distribution and pharmacological characterization of a novel gonadotropin-releasing hormone ([Trp8] GnRH) in frog brain [J].
Yoo, MS ;
Kang, HM ;
Choi, HS ;
Kim, JW ;
Troskie, BE ;
Millar, RP ;
Kwon, HB .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2000, 164 (1-2) :197-204
[29]   Stressor-responsive central nesfatin-1 activates corticotropin-releasing hormone, noradrenaline and serotonin neurons and evokes hypothalamic-pituitary-adrenal axis [J].
Yoshida, Natsu ;
Maejima, Yuko ;
Sedbazar, Udval ;
Ando, Akihiko ;
Kurita, Hideharu ;
Damdindorj, Boldbaatar ;
Takano, Eisuke ;
Gantulga, Darambazar ;
Iwasaki, Yusaku ;
Kurashina, Tomoyuki ;
Onaka, Tatsushi ;
Dezaki, Katsuya ;
Nakata, Masanori ;
Mori, Masatomo ;
Yada, Toshihiko .
AGING-US, 2010, 2 (11) :775-784
[30]   Amphibian larvae and zinc sulphate: a suitable model to study the role of brain-derived neurotrophic factor (BDNF) in the neuronal turnover of the olfactory epithelium [J].
Yovanovich, Carola A. M. ;
Jungblut, Lucas D. ;
Heer, Tamara ;
Pozzi, Andrea G. ;
Paz, Dante A. .
CELL AND TISSUE RESEARCH, 2009, 336 (01) :1-9
123