In vivo fluorescence imaging with high-resolution microlenses

被引：199

作者：

Barretto, Robert P. J. ^{[1
]}

Messerschmidt, Bernhard ^{[2
]}

Schnitzer, Mark J. ^{[1
,3
]}

机构：

[1] Stanford Univ, James H Clark Ctr Biomed Engn & Sci, Stanford, CA 94305 USA

[2] GRINTECH GmbH, Jena, Germany

[3] Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA

来源：

NATURE METHODS | 2009年 / 6卷 / 07期

基金：

美国国家卫生研究院;

关键词：

MICROOPTIC LENSES; ION-EXCHANGE; HIGH-SPEED; MICROENDOSCOPY; MICROSCOPY; MICE;

D O I：

10.1038/nmeth.1339

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Micro-optics are increasingly used for minimally invasive in vivo imaging, in miniaturized microscopes and in lab-on-a-chip devices. Owing to optical aberrations and lower numerical apertures, a main class of microlens, gradient refractive index lenses, has not achieved resolution comparable to conventional microscopy. Here we describe high-resolution microlenses, and illustrate two-photon imaging of dendritic spines on hippocampal neurons and dual-color nonlinear optical imaging of neuromuscular junctions in live mice.

引用

页码：511 / U61

页数：4

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