Transformation of membrane nanosurface of red blood cells under hemin action

被引：23
作者：
Kozlova, Elena [1 ]
Chernysh, Alexander [1 ]
Moroz, Victor [1 ]
Gudkova, Olga [1 ]
Sergunova, Victoria [1 ]
Kuzovlev, Artem [1 ]
机构：
[1] RAS, VA Negovsky Sci Res Inst Gen Reanimatol, Moscow 117901, Russia
来源：
SCIENTIFIC REPORTS | 2014年 / 4卷
关键词：
ATOMIC-FORCE MICROSCOPY; ERYTHROCYTE-MEMBRANES; OXIDATION; ELECTROPORATION; PROTEIN; STATE;
D O I：
10.1038/srep06033
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Hemin is the product of hemoglobin oxidation. Some diseases may lead to a formation of hemin. The accumulation of hemin causes destruction of red blood cells (RBC) membranes. In this study the process of development of topological defects of RBC membranes within the size range from nanoscale to microscale levels is shown. The formation of the grain-like structures in the membrane ("grains'') with typical sizes of 120-200 nm was experimentally shown. The process of formation of "grains'' was dependent on the hemin concentration and incubation time. The possible mechanism of membrane nanostructure alterations is proposed. The kinetic equations of formation and transformation of small and medium topological defects were analyzed. This research can be used to study the cell intoxication and analyze the action of various agents on RBC membranes.
引用
收藏
页数：11
相关论文
共 34 条
  • [1] BECKER PS, 1986, J BIOL CHEM, V261, P4620
  • [2] Heme Degradation and Vascular Injury
    Belcher, John D.
    Beckman, Joan D.
    Balla, Gyorgy
    Balla, Jozsef
    Vercellotti, Gregory
    [J]. ANTIOXIDANTS & REDOX SIGNALING, 2010, 12 (02) : 233 - 248
  • [3] Control of Erythrocyte Membrane-Skeletal Cohesion by the Spectrin-Membrane Linkage
    Blanc, Lionel
    Salomao, Marcela
    Guo, Xinhua
    An, Xiuli
    Gratzer, Walter
    Mohandas, Narla
    [J]. BIOCHEMISTRY, 2010, 49 (21) : 4516 - 4523
  • [4] Reversible Zinc-Induced Injuries to Erythrocyte Membrane Nanostructure
    Chernysh, A. M.
    Kozlova, E. K.
    Moroz, V. V.
    Sergunova, V. A.
    Gudkova, O. Ye.
    Fedorova, M. S.
    [J]. BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE, 2012, 154 (01) : 84 - 88
  • [5] Erythrocyte Membrane Surface after Calibrated Electroporation: Visualization by Atomic Force Microscopy
    Chernysh, A. M.
    Kozlova, E. K.
    Moroz, V. V.
    Borshagovskaya, P. Yu.
    Bliznuk, U. A.
    Rysaeva, R. M.
    [J]. BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE, 2009, 148 (03) : 455 - 460
  • [6] Hemin-induced membrane sulfhydryl oxidation: Possible involvement of thiyl radicals
    Chiu, DTY
    Huang, TY
    Hung, IJ
    Wei, JS
    Liu, TZ
    Stern, A
    [J]. FREE RADICAL RESEARCH, 1997, 27 (01) : 55 - 62
  • [7] Hereditary spherocytosis - Defects in proteins that connect the membrane skeleton to the lipid bilayer
    Eber, S
    Lux, SE
    [J]. SEMINARS IN HEMATOLOGY, 2004, 41 (02) : 118 - 141
  • [8] Hemin-induced suicidal erythrocyte death
    Gatidis, Sergios
    Foeller, Michael
    Lang, Florian
    [J]. ANNALS OF HEMATOLOGY, 2009, 88 (08) : 721 - 726
  • [9] Roughness of the plasma membrane as an independent morphological parameter to study RBCs: A quantitative atomic force microscopy investigation
    Girasole, M.
    Pompeo, G.
    Cricenti, A.
    Congiu-Castellano, A.
    Andreola, F.
    Serafino, A.
    Frazer, B. H.
    Boumis, G.
    Amiconi, G.
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2007, 1768 (05): : 1268 - 1276
  • [10] Red blood cell membrane fluctuations and shape controlled by ATP-induced cytoskeletal defects
    Gov, NS
    Safran, SA
    [J]. BIOPHYSICAL JOURNAL, 2005, 88 (03) : 1859 - 1874
1234