Transcriptomic microarray analysis of BoMac cells after infection with bovine foamy virus

被引：2
作者：
Rola-Luszczak, Marzena [1 ]
Materniak, Magdalena [1 ]
Pluta, Aneta [1 ]
Hulst, Marcel [2 ]
Kuzmak, Jacek [1 ]
机构：
[1] Natl Inst Vet Res, Dept Biochem, PL-24100 Pulawy, Poland
[2] Livestock Res Wageningen Univ & Res Ctr, NL-8200 AB Lelystad, Netherlands
来源：
ARCHIVES OF VIROLOGY | 2014年 / 159卷 / 06期
关键词：
Bovine foamy virus; Microarray; Bovine macrophage cell line; LEUKEMIA-VIRUS; PERIPHERAL-BLOOD; GENE-EXPRESSION; SYNCYTIAL VIRUS; UP-REGULATION; CATTLE; IDENTIFICATION; APOBEC3; LYMPHOCYTES; TAXILIN;
D O I：
10.1007/s00705-013-1959-1
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Bovine foamy virus (BFV) infections are highly prevalent among cattle worldwide. However, relatively little is known about the impact of this virus on the host immune system. In our study, we focused on a bovine macrophage cell line (BoMac) and examined changes in the BoMac transcriptome after in vitro infection with BFV using bovine BLOPlus oligo microarrays. One hundred twenty-four genes showed significant changes in expression level. The biological process categories found to be enriched include metabolic processes, cell communication, transport, immune system processes, and response to extracellular stimuli. RT-qPCR was applied to confirm the results obtained for representative genes.
引用
收藏
页码：1515 / 1519
页数：5
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