Microarray for molecular typing of Salmonella enterica serovars

被引：42

作者：

Scaria, Joy ^{[1
]}

Palaniappan, Raghavan U. M. ^{[1
]}

Chiu, David ^{[1
]}

Phan, Julie Ann ^{[1
]}

Ponnala, Lalit ^{[2
]}

McDonough, Patrick ^{[1
]}

Grohn, Yrjo T. ^{[1
]}

Porwollik, Steffen ^{[3
]}

McClelland, Michael ^{[3
]}

Chiou, Chien-Shun

Chu, Chishih ^{[4
]}

Chang, Yung-Fu ^{[1
]}

机构：

[1] Cornell Univ, Dept Populat Med & Diagnost Sci, Coll Vet Med, Ithaca, NY 14853 USA

[2] Cornell Univ, Ctr Adv Comp, Ithaca, NY 14853 USA

[3] Sidney Kimmel Canc Ctr, San Diego, CA 92121 USA

[4] Natl Chiayi Univ, Dept Microbiol & Immunol, Chiayi, Taiwan

来源：

MOLECULAR AND CELLULAR PROBES | 2008年 / 22卷 / 04期

关键词：

Salmonella; Microarray; Oligonucleotide;

D O I：

10.1016/j.mcp.2008.04.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the development of a spotted array for the delineation of the most common 14 disease-causing Salmonella serovars in the United States. Our array consists of 414 70 mers targeting core genes of Salmonella enterica, subspecies I specific genes, fimbrial genes, pathogenicity islands, Gifsy elements and other variable genes. Using this array we were able to identify a unique gene presence/absence profile for each of the targeted serovar which was used as the serovar differentiating criteria. Based on this profile, we developed a Matlab programme that compares the profile of an unknown sample to all 14 reference serovar profiles and give out the closest serovar match. Since we have included probes targeting most of the virulence genes and variable genes in Salmonella, in addition to using for serovar detection this array could also be used for studying the virulence gene content and also for evaluating the genetic relation between different isolates of Salmonella. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：238 / 243

页数：6

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