Cell immersion and cell dipping in microfluidic devices

被引：76

作者：

Seger, U ^{[1
]}

Gawad, S ^{[1
]}

Johann, R ^{[1
]}

Bertsch, A ^{[1
]}

Renaud, P ^{[1
]}

机构：

[1] Swiss Fed Inst Technol, EPFL, STI, LMIS, CH-1015 Lausanne, Switzerland

来源：

LAB ON A CHIP | 2004年 / 4卷 / 02期

关键词：

D O I：

10.1039/b311210a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Combining deflective dielectrophoretic barriers with controlled pressure driven liquid flows in microfluidic devices allows accurate handling of particles such as biological cells in suspensions. Working towards cell-based lab-on-a-chip applications, a platform permitting rapid testing of devices having different dielectrophoretic and fluidic subunits was developed. The performance of such a system is shown in the cases of (A) flooding a small number of immobilised cells with a dye and (B) transient buffer swapping of a large number of cells in flow. The transition times for moving cells from one reagent to the other are below 0.5 s in the case of flow-through cell dipping.

引用

页码：148 / 151

页数：4

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