Ablation of Runx2 in Ameloblasts Suppresses Enamel Maturation in Tooth Development

被引:28
作者
Chu, Qing [1 ]
Gao, Yan [1 ]
Gao, Xianhua [1 ]
Dong, Zhiheng [1 ]
Song, Wenying [1 ]
Xu, Zhenzhen [1 ]
Xiang, Lili [1 ]
Wang, Yumin [2 ]
Zhang, Li [2 ]
Li, Mingyu [3 ]
Gao, Yuguang [1 ]
机构
[1] Binzhou Med Univ, Dept Pediat & Prevent Dent, Binzhou 256600, Shandong, Peoples R China
[2] Binzhou Med Univ, Inst Stomatol, Yantai 255000, Shandong, Peoples R China
[3] Shanghai Jiao Tong Univ, Peoples Hosp 9, Fac Med, Shanghai Key Lab Stomatol,Shanghai Res Inst Stom, Shanghai 200011, Peoples R China
基金
中国国家自然科学基金;
关键词
TRANSCRIPTION FACTOR-2 RUNX2; AMELOGENESIS IMPERFECTA; CLEIDOCRANIAL DYSPLASIA; MATRIX PROTEINS; GENE-EXPRESSION; MUTATION; MMP20; AMELOTIN; DEFECTS; CBFA1;
D O I
10.1038/s41598-018-27873-5
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Runt-related transcription factor 2 (Runx2) is involved in the early stage of tooth development. However, only few studies have reported the role of Runx2 in enamel development, which may be attributed to that Runx2 full knockout mice cannot survive after birth. In the present study, we successfully established a Runx2-deficient mouse model using a conditional knockout (cKO) method. We observed a significant reduction in the degree of mineralization and the decreased size of enamel rods in cKO mice. Histological analysis showed the retained enamel proteins in enamel layer at maturation stage in cKO molars. Further analysis by qRT-PCR revealed that the expressions of genes encoding enamel structure proteins, such as amelogenin (AMELX), ameloblastin (AMBN) and enamelin (ENAM), were increased in cKO enamel organs. On the other hand, the expression of kallikrein-related peptidase-4 (KLK4) at the mRNA and protein levels was dramatically decreased from late secretory stage to maturation stage in cKO enamel organs, while the expression of matrix metalloproteinase-20 (MMP-20) was not significantly altered. Finally, immunohistochemistry indicated that the uptake of amelogenins by ameloblasts was significantly decreased in cKO mice. Taken together, Runx2 played critical roles in controlling enamel maturation by increasing synthesis of KLK4 and decreasing synthesis of AMELX, AMBN and ENAM.
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页数:10
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