A dietary combination of omega-3 and omega-6 polyunsaturated fatty acids is more efficient than single supplementations in the prevention of retinal damage induced by elevation of intraocular pressure in rats

被引:49
作者
Schnebelen, Coralie [1 ]
Pasquis, Bruno [1 ]
Salinas-Navarro, Manuel [2 ]
Joffre, Corinne [1 ]
Creuzot-Garcher, Catherine P. [1 ]
Vidal-Sanz, Manuel [2 ]
Bron, Alain M. [1 ]
Bretillon, Lionel [1 ]
Acar, Niyazi [1 ]
机构
[1] INRA, UMR1129, FLAVIC, Eye & Nutr Res Grp, F-21000 Dijon, France
[2] Univ Murcia, Dept Ophthalmol, E-30100 Murcia, Spain
关键词
Dietary polyunsaturated fatty acids; Omega-3; Omega-6; Elevated IOP; Rat; GAMMA-LINOLENIC ACID; GANGLION-CELL DEATH; NECROSIS-FACTOR-ALPHA; OPEN-ANGLE GLAUCOMA; DOCOSAHEXAENOIC ACID; OPTIC-NERVE; LASER PHOTOCOAGULATION; MACULAR DEGENERATION; ISCHEMIA-REPERFUSION; OCULAR HYPERTENSION;
D O I
10.1007/s00417-009-1094-6
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
To evaluate the effect of a dietary combination of omega-3 and omega-6 polyunsaturated fatty acids (PUFAs) compared to single PUFA supplementations on the outcome of a substantial elevation of intraocular pressure (IOP) in rats. Sprague Dawley rats were fed for 6 months with either a control diet, a diet enriched with omega-3 PUFAs (eicosapentaenoic acid, EPA, and docosahexaenoic acid, DHA), a diet enriched with omega-6 PUFAs (gamma-linolenic acid, GLA) or a diet enriched with both omega-3 and omega-6 PUFAs (EPA + DHA and GLA). After 3 months of feeding, elevation of IOP was induced by photocoagulation of the episcleral veins, limbus and trabecular meshwork using a 532-nm laser. IOP and scotopic electroretinograms (ERGs) were monitored after the induction of IOP elevation until the end of the nutritional supplementation. Retinal morphometry and GFAP immunohistochemistry were performed 3 months after laser photocoagulation. Retinal ganglion cells (RGCs) were quantified using retrograde labelling. A significant rise in IOP was observed in the laser-treated eyes. PUFA supplementation did not influence the time course of IOP in the laser-treated eyes. Three months after laser photocoagulation, the activation of glial cells observed in the laser-treated eyes was significantly lower in animals fed with the EPA + DHA + GLA diet when compared to those fed the control diet, while single supplementations with either EPA + DHA or GLA were not effective. The same protective effect of the EPA + DHA + GLA combination was observed on retinal structures in the laser-treated eyes. However, PUFA supplementation did not influence either ERG b-wave amplitude or the RGC loss in the laser-treated eyes. This study demonstrates that a 6-month supplementation with a combination of omega-3 and omega-6 PUFAs is more effective than single supplementations, since the EPA + DHA + GLA dietary combination prevented retinal cell structure and decreased glial cell activation induced by the elevation of IOP in rats.
引用
收藏
页码:1191 / 1203
页数:13
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