Production of pigment-free pullulan by swollen cell in Aureobasidium pullulans NG which cell differentiation was affected by pH and nutrition

被引:45
作者
Li, Bing-xue [1 ,2 ,3 ]
Zhang, Ning [4 ]
Peng, Qing [1 ,2 ]
Yin, Tie [1 ,2 ]
Guan, Fei-fei [1 ,2 ]
Wang, Gui-li [1 ,2 ]
Li, Ying [1 ,2 ]
机构
[1] China Agr Univ, State Key Labs Agrobiotechnol, Beijing 100193, Peoples R China
[2] China Agr Univ, Coll Biol Sci, Beijing 100193, Peoples R China
[3] Shenyang Agr Univ, Coll Soil & Environm Sci, Microbiol Lab, Shenyang 110161, Peoples R China
[4] Shenyang Agr Univ, Coll Biol Sci & Technol, Dev Biol Lab, Shenyang 110161, Peoples R China
关键词
Aureobasidium pullulans; Swollen cell; pH; Pullulan; Melanin; MOLECULAR-WEIGHT PULLULAN; REDUCED PIGMENTATION; EXTRACELLULAR POLYSACCHARIDE; YEAST-LIKE; EXOPOLYSACCHARIDE; ELABORATION; TRANSITION; GROWTH; MUTANT;
D O I
10.1007/s00253-009-1955-2
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A black yeast strain "NG" was isolated from strawberry fruit and identified as Aureobasidium pullulans. Strain NG displayed yeast-like cell (YL), swollen cell (SC), septate swollen cell (SSC), meristematic structure (MS), and chlamydospore (CH) morphologies. pH was the key factor regulating cell morphogenesis of strain NG. Differentiation of YL controlled by extracellular pH had no relationship with nutrition level. YL was maintained at pH > 6.0, but was transformed into SC at pH similar to 4.5. SC, a stable cell type of A. pullulans, could bud, septate, or transform into MS or CH, in response to nutrition level and low pH. SC produced swollen cell blastospores (SCB) at pH 2.1 with abundant nutrition, and could transform into MS at lower pH (1.5). SC was induced to form CH by low level nutrition and pH < 3, and this transition was suppressed by adjusting pH to similar to 4.5. Crude polysaccharides without pigment (melanin) were produced by SC of strain NG. Pullulan content of the polysaccharides was very high (98.37%). Fourier-transform infrared spectroscopy confirmed that chemical structures of the polysaccharides and standard pullulan were identical. Swollen cells produced 2.08 mg/ml non-pigmented polysaccharides at 96 h in YPD medium. Controlling pH of fermentation is an effective and convenient method to harvest SC for melanin-free pullulan production.
引用
收藏
页码:293 / 300
页数:8
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