Absolute bioluminescence imaging at the single-cell level with a light signal at the Attowatt level

被引：12

作者：

Enomoto, Toshiteru ^{[1
]}

Kubota, Hidehiro ^{[1
]}

Mori, Kaneo ^{[1
]}

Shimogawara, Masahiro ^{[1
]}

Yoshita, Masahiro ^{[2
]}

Ohmiya, Yoshihiro ^{[3
,4
]}

Akiyama, Hidefumi ^{[4
,5
]}

机构：

[1] ATTO Corp, Taito Ku, 3-2-2 Motoasakusa, Tokyo 1110041, Japan

[2] Natl Inst Adv Ind Sci & Technol, 1-1-1 Umezono, Tsukuba, Ibaraki 3058568, Japan

[3] Natl Inst Adv Ind Sci & Technol, DAILAB, 1-1-1 Higashi, Tsukuba, Ibaraki 3058568, Japan

[4] AIST UTokyo OPERANDO OIL, Kashiwa, Chiba 2778581, Japan

[5] Univ Tokyo, ISSP, 5-1-5 Kashiwanoha, Kashiwa, Chiba 2778581, Japan

来源：

BIOTECHNIQUES | 2018年 / 64卷 / 06期

关键词：

beetle luciferase; bioluminescence imaging; single-cell level; BEETLE LUCIFERASES; GENE-EXPRESSION; ORGANISMS; EMISSION;

D O I：

10.2144/btn-2018-0043

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bioluminescence imaging (BLI) demonstrates cellular events as a light signal at the single-cell level using a highly sensitive, cooled CCD camera. However, BLI signals are relative values and thus, images taken on different days or using different equipment cannot be compared directly. We established a reference LED light source that was characteristic of the total flux and light distribution and calibrated the BLI system as an absolute light signal. This calibrated BLI system revealed that the average light signal of beetle luciferase was at an attowatt level per sec at the single cell level.

引用

页码：270 / 274

页数：5

共 12 条

[1] Use of reporter genes for optical measurements of neoplastic disease in vivo [J].

Contag, CH ;

Jenkins, D ;

Contag, FR ;

Negrin, RS .

NEOPLASIA, 2000, 2 (1-2) :41-52

[2] Imaging of light emission from the expression of luciferases in living cells and organisms: a review [J].

Greer, LF ;

Szalay, AA .

LUMINESCENCE, 2002, 17 (01) :43-74

[3] Luciferase-YFP fusion tag with enhanced emission for single-cell luminescence imaging [J].

Hoshino, Hideto ;

Nakajima, Yoshihiro ;

Ohmiya, Yoshihiro .

NATURE METHODS, 2007, 4 (08) :637-639

[4] Bioluminescence Microscopy as a Method to Measure Single Cell Androgen Receptor Activity Heterogeneous Responses to Antiandrogens [J].

Jain, Pallavi ;

Neveu, Bertrand ;

Velot, Lauriane ;

Wu, Lily ;

Fradet, Yves ;

Pouliot, Frederic .

SCIENTIFIC REPORTS, 2016, 6

[5] Bioluminescence imaging of dual gene expression at the single-cell level [J].

Kwon, HyuckJoon ;

Enomoto, Toshiteru ;

Shimogawara, Masahiro ;

Yasuda, Kazunori ;

Nakajima, Yoshihiro ;

Ohmiya, Yoshihiro .

BIOTECHNIQUES, 2010, 48 (06) :460-462

[6] A Single-Cell Bioluminescence Imaging System for Monitoring Cellular Gene Expression in a Plant Body [J].

Muranaka, Tomoaki ;

Kubota, Saya ;

Oyama, Tokitaka .

PLANT AND CELL PHYSIOLOGY, 2013, 54 (12) :2085-2093

[7] Enhanced Beetle Luciferase for High-Resolution Bioluminescence Imaging [J].

Nakajima, Yoshihiro ;

Yamazaki, Tomomi ;

Nishii, Shigeaki ;

Noguchi, Takako ;

Hoshino, Hideto ;

Niwa, Kazuki ;

Viviani, Vadim R. ;

Ohmiya, Yoshihiro .

PLOS ONE, 2010, 5 (03)

[8] Quantum Yields and Kinetics of the Firefly Bioluminescence Reaction of Beetle Luciferases [J].

Niwa, Kazuki ;

Ichino, Yoshiro ;

Kumata, Shiho ;

Nakajima, Yoshihiro ;

Hiraishi, Yoshihiro ;

Kato, Dai-ichiro ;

Viviani, Vadim R. ;

Ohmiya, Yoshihiro .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 2010, 86 (05) :1046-1049

[9] Simultaneous Multicolor Luciferase Reporter Assays for Monitoring of Multiple Genes Expressions [J].

Ohmiya, Yoshihiro .

COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING, 2015, 18 (10) :937-945

[10] Bioluminescence imaging in living organisms [J].

Welsh, DK ;

Kay, SA .

CURRENT OPINION IN BIOTECHNOLOGY, 2005, 16 (01) :73-78

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